biostudies-literaturePanas MWHHS | National Institutes of HealthHuman Frontier Science ProgramNIAID NIH HHSNCI NIH HHSNational Science Foundatione00674-19https://www.ebi.ac.uk/biostudies/studies/S-EPMC6495377biostudies-literatureUnknown10(2)Toxoplasma gondii is an obligate intracellular parasite that establishes a favorable environment in the host cells in which it replicates. We have previously reported that it uses MYR-dependent translocation of dense granule proteins to elicit a key set of host responses related to the cell cycle, specifically, E2F transcription factor targets, including cyclin E. We report here the identification of a novel Toxoplasma effector protein that is exported from the parasitophorous vacuole in a MYR1-dependent manner and localizes to the host's nucleus. Parasites lacking this inducer of host cyclin E (HCE1) are unable to modulate E2F transcription factor target genes and exhibit a substantial growth defect. Immunoprecipitation of HCE1 from infected host cells smBioToxoplasma Controls Host Cyclin E Expression through the Use of a Novel MYR1-Dependent Effector Protein, HCE1.PMC6495377R01 AI021423P30 CA124435RO1-AI021423LT000404/2014-LR01 AI129529RO1-AI129529DGE-114747Panas MWNaor ACygan AMBoothroyd JCfalseToxoplasma Controls Host Cyclin E Expression through the Use of a Novel MYR1-Dependent Effector Protein, HCE1.Toxoplasma gondii is an obligate intracellular parasite that establishes a favorable environment in the host cells in which it replicates. We have previously reported that it uses MYR-dependent translocation of dense granule proteins to elicit a key set of host responses related to the cell cycle, specifically, E2F transcription factor targets, including cyclin E. We report here the identification of a novel Toxoplasma effector protein that is exported from the parasitophorous vacuole in a MYR1-dependent manner and localizes to the host's nucleus. Parasites lacking this inducer of host cyclin E (HCE1) are unable to modulate E2F transcription factor target genes and exhibit a substantial growth defect. Immunoprecipitation of HCE1 from infected host cells s2019-01-01T00:00:00Z2019 Apr2026-06-16T07:04:30.523Z2019-06-06T23:10:28ZS-EPMC64953773104024210.1128/mBio.00674-19