{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu S"],"funding":["National Natural Science Foundation of China"],"pagination":["6574-6583"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6497428"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(5)"],"pubmed_abstract":["Mutations may ultimately change the local conformation of proteins; however, little attention has been paid to alterations in protein function caused by the incidence of <i>cis</i>-peptide bonds (ICPB) in mammalian cells. In this study, a statistical approach, coimmunoprecipitation, and immunofluorescence staining have been used to confirm that S246→Y and S246→W missense mutations, which help increase the ICPB in Xaa246-P247 (Xaa is any amino acid) in human β-catenin, can reduce the interactions between β-catenin and adenomatous polyposis coli (APC) and between β-catenin and Ca2<sup>+</sup>-dependent cell adhesion molecule family in epithelial tissue (E-cadherin), eventually leading to increased nuclear migration of β-catenin in the HepG2 cell line (an immortalized cell line consisting of "],"journal":["FASEB journal : official publication of the Federation of American Societies for Experimental Biology"],"pubmed_title":["Subcellular localization of mutated β-catenins with different incidences of <i>cis</i>-peptide bonds at the Xaa246-P247 site in HepG2 cells."],"pmcid":["PMC6497428"],"funding_grant_id":["31501100"],"pubmed_authors":["Yang H","Yang Y","Yu S","Zhang Z","Zhang Y","Chen Y","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Subcellular localization of mutated β-catenins with different incidences of <i>cis</i>-peptide bonds at the Xaa246-P247 site in HepG2 cells.","description":"Mutations may ultimately change the local conformation of proteins; however, little attention has been paid to alterations in protein function caused by the incidence of <i>cis</i>-peptide bonds (ICPB) in mammalian cells. In this study, a statistical approach, coimmunoprecipitation, and immunofluorescence staining have been used to confirm that S246→Y and S246→W missense mutations, which help increase the ICPB in Xaa246-P247 (Xaa is any amino acid) in human β-catenin, can reduce the interactions between β-catenin and adenomatous polyposis coli (APC) and between β-catenin and Ca2<sup>+</sup>-dependent cell adhesion molecule family in epithelial tissue (E-cadherin), eventually leading to increased nuclear migration of β-catenin in the HepG2 cell line (an immortalized cell line consisting of ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2026-04-14T13:39:00.59Z","creation":"2019-06-06T23:10:35Z"},"accession":"S-EPMC6497428","cross_references":{"pubmed":["30807699"],"doi":["10.1096/fj.201801937RR"]}}