biostudies-literatureYu SNational Natural Science Foundation of China6574-6583https://www.ebi.ac.uk/biostudies/studies/S-EPMC6497428biostudies-literatureUnknown33(5)Mutations may ultimately change the local conformation of proteins; however, little attention has been paid to alterations in protein function caused by the incidence of <i>cis</i>-peptide bonds (ICPB) in mammalian cells. In this study, a statistical approach, coimmunoprecipitation, and immunofluorescence staining have been used to confirm that S246→Y and S246→W missense mutations, which help increase the ICPB in Xaa246-P247 (Xaa is any amino acid) in human β-catenin, can reduce the interactions between β-catenin and adenomatous polyposis coli (APC) and between β-catenin and Ca2⁺-dependent cell adhesion molecule family in epithelial tissue (E-cadherin), eventually leading to increased nuclear migration of β-catenin in the HepG2 cell line (an immortalized cell line consisting of FASEB journal : official publication of the Federation of American Societies for Experimental BiologySubcellular localization of mutated β-catenins with different incidences of <i>cis</i>-peptide bonds at the Xaa246-P247 site in HepG2 cells.PMC649742831501100Yang HYang YYu SZhang ZZhang YChen YWu YfalseSubcellular localization of mutated β-catenins with different incidences of <i>cis</i>-peptide bonds at the Xaa246-P247 site in HepG2 cells.Mutations may ultimately change the local conformation of proteins; however, little attention has been paid to alterations in protein function caused by the incidence of <i>cis</i>-peptide bonds (ICPB) in mammalian cells. In this study, a statistical approach, coimmunoprecipitation, and immunofluorescence staining have been used to confirm that S246→Y and S246→W missense mutations, which help increase the ICPB in Xaa246-P247 (Xaa is any amino acid) in human β-catenin, can reduce the interactions between β-catenin and adenomatous polyposis coli (APC) and between β-catenin and Ca2⁺-dependent cell adhesion molecule family in epithelial tissue (E-cadherin), eventually leading to increased nuclear migration of β-catenin in the HepG2 cell line (an immortalized cell line consisting of 2019-01-01T00:00:00Z2019 May2026-04-14T13:39:00.59Z2019-06-06T23:10:35ZS-EPMC64974283080769910.1096/fj.201801937RR