biostudies-literatureUnknown9(1)Itaba NChronic hepatitis viral infection, alcoholic intoxication, and obesity cause liver fibrosis, which progresses to decompensated liver cirrhosis, a disease for which medical demands cannot be met. Since there are currently no approved anti-fibrotic therapies for established liver fibrosis, the development of novel modalities is required to improve patient prognosis. In this study, we clarified the anti-fibrotic effects of cell sheets produced from human bone marrow-derived mesenchymal stem cells (MSCs) incubated on a temperature-sensitive culture dish with the chemical compound IC-2. Orthotopic transplantation of IC-2-engineered MSC sheets (IC-2 sheets) remarkably reduced liver fibrosis induced by chronic CCl₄ administration. Further, the marked production of fibrolytic enzymes suScientific reports6841https://www.ebi.ac.uk/biostudies/studies/S-EPMC6497888biostudies-literatureReversal of established liver fibrosis by IC-2-engineered mesenchymal stem cell sheets.PMC6497888Kono YOka HYokobata TKakuta HShiota GMorimoto MWatanabe KOsaki MItaba NfalseReversal of established liver fibrosis by IC-2-engineered mesenchymal stem cell sheets.Chronic hepatitis viral infection, alcoholic intoxication, and obesity cause liver fibrosis, which progresses to decompensated liver cirrhosis, a disease for which medical demands cannot be met. Since there are currently no approved anti-fibrotic therapies for established liver fibrosis, the development of novel modalities is required to improve patient prognosis. In this study, we clarified the anti-fibrotic effects of cell sheets produced from human bone marrow-derived mesenchymal stem cells (MSCs) incubated on a temperature-sensitive culture dish with the chemical compound IC-2. Orthotopic transplantation of IC-2-engineered MSC sheets (IC-2 sheets) remarkably reduced liver fibrosis induced by chronic CCl₄ administration. Further, the marked production of fibrolytic enzymes su2019-01-01T00:00:00Z2019 May2025-04-18T15:28:05.976Z2019-06-06T23:26:45ZS-EPMC64978883104874010.1038/s41598-019-43298-0