biostudies-literatureRosso MConsejo Nacional de Investigaciones Científicas y TécnicasFundación Alberto J. RoemmersFP7 People: Marie-Curie ActionsInstituto Nacional del CáncerAgencia Nacional de Promoción Científica y Tecnológica5https://www.ebi.ac.uk/biostudies/studies/S-EPMC6507066biostudies-literatureUnknown7<h4>Background</h4>Breast cancer (BC) is the most common female cancer and the leading cause of cancer death in women worldwide. Alterations in epithelial cadherin (E-cadherin) expression and functions are associated to BC, but the underlying molecular mechanisms have not been fully elucidated. We have previously reported a novel human E-cadherin splice variant (E-cadherin variant) mRNA. Stable transfectants in MCF-7 human BC cells (MCF7Ecadvar) depicted fibroblast-like cell morphology, E-cadherin wild-type downregulation, and other molecular changes characteristic of the epithelial-to-mesenchymal transition process, reduced cell-cell adhesion, and increased cell migration and invasion. In this study, a two-dimensional differential gel electrophoresis (2D-DIGE) combined with mass spectromeCancer & metabolismCharacterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an increased expression of lactate dehydrogenase B.PMC65070662016-2017PIP7402011-2013PICT SU 10722014-2015PIP887269285Matos MLBesso MJReventos JCanals FQuevedo Cuenca JORosso MVazquez-Levin MHLapyckyj LMonge MfalseCharacterization of the molecular changes associated with the overexpression of a novel epithelial cadherin splice variant mRNA in a breast cancer model using proteomics and bioinformatics approaches: identification of changes in cell metabolism and an increased expression of lactate dehydrogenase B.<h4>Background</h4>Breast cancer (BC) is the most common female cancer and the leading cause of cancer death in women worldwide. Alterations in epithelial cadherin (E-cadherin) expression and functions are associated to BC, but the underlying molecular mechanisms have not been fully elucidated. We have previously reported a novel human E-cadherin splice variant (E-cadherin variant) mRNA. Stable transfectants in MCF-7 human BC cells (MCF7Ecadvar) depicted fibroblast-like cell morphology, E-cadherin wild-type downregulation, and other molecular changes characteristic of the epithelial-to-mesenchymal transition process, reduced cell-cell adhesion, and increased cell migration and invasion. In this study, a two-dimensional differential gel electrophoresis (2D-DIGE) combined with mass spectrome2019-01-01T00:00:00Z20192026-05-07T02:14:56.396Z2019-06-06T23:29:04ZS-EPMC65070663108665910.1186/s40170-019-0196-9