{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["31(2)"],"submitter":["Xu X"],"pubmed_abstract":["<h4>Objective</h4>Epidermal growth factor receptor (EGFR) is overexpressed in a wide variety of solid tumors, serving as a well-characterized target for cancer imaging or therapy. In this study, we aimed to design and synthesize a radiotracer, <sup>64</sup>Cu-NOTA-C225, targeting EGFR for tumor positron emission tomography (PET) imaging.<h4>Methods</h4>Cetuximab (C225) was conjugated to a bifunctional chelator, p-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and further radiolabeled with copper-64 for PET imaging. <sup>64</sup>Cu-NOTA-IgG and Cy5.5-C225 were also synthesized as control probes. A431 and A549 mouse models were established for micro-PET and/or near-infrared fluorescence (NIRF) imaging.<h4>Results</h4><sup>64</sup>Cu-NOTA-C225 exhibited stability <i"],"journal":["Chinese journal of cancer research = Chung-kuo yen cheng yen chiu"],"pagination":["400-409"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6513748"],"repository":["biostudies-literature"],"pubmed_title":["Synthesis and evaluation of <sup>64</sup>Cu-radiolabeled NOTA-cetuximab (<sup>64</sup>Cu-NOTA-C225) for immuno-PET imaging of EGFR expression."],"pmcid":["PMC6513748"],"pubmed_authors":["Yang X","Yang Z","Xia L","Guo X","Huang H","Zhu H","Xu X","Liu T","Xie Q","Liu F","Li N","Xin Y"],"additional_accession":[]},"is_claimable":false,"name":"Synthesis and evaluation of <sup>64</sup>Cu-radiolabeled NOTA-cetuximab (<sup>64</sup>Cu-NOTA-C225) for immuno-PET imaging of EGFR expression.","description":"<h4>Objective</h4>Epidermal growth factor receptor (EGFR) is overexpressed in a wide variety of solid tumors, serving as a well-characterized target for cancer imaging or therapy. In this study, we aimed to design and synthesize a radiotracer, <sup>64</sup>Cu-NOTA-C225, targeting EGFR for tumor positron emission tomography (PET) imaging.<h4>Methods</h4>Cetuximab (C225) was conjugated to a bifunctional chelator, p-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and further radiolabeled with copper-64 for PET imaging. <sup>64</sup>Cu-NOTA-IgG and Cy5.5-C225 were also synthesized as control probes. A431 and A549 mouse models were established for micro-PET and/or near-infrared fluorescence (NIRF) imaging.<h4>Results</h4><sup>64</sup>Cu-NOTA-C225 exhibited stability <i","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2026-06-01T06:32:31.43Z","creation":"2026-04-08T10:01:33.95Z"},"accession":"S-EPMC6513748","cross_references":{"pubmed":["31156310"],"doi":["10.21147/j.issn.1000-9604.2019.02.14"]}}