biostudies-literatureUnknown31(2)Xu X<h4>Objective</h4>Epidermal growth factor receptor (EGFR) is overexpressed in a wide variety of solid tumors, serving as a well-characterized target for cancer imaging or therapy. In this study, we aimed to design and synthesize a radiotracer, ⁶⁴Cu-NOTA-C225, targeting EGFR for tumor positron emission tomography (PET) imaging.<h4>Methods</h4>Cetuximab (C225) was conjugated to a bifunctional chelator, p-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and further radiolabeled with copper-64 for PET imaging. ⁶⁴Cu-NOTA-IgG and Cy5.5-C225 were also synthesized as control probes. A431 and A549 mouse models were established for micro-PET and/or near-infrared fluorescence (NIRF) imaging.<h4>Results</h4>⁶⁴Cu-NOTA-C225 exhibited stability <iChinese journal of cancer research = Chung-kuo yen cheng yen chiu400-409https://www.ebi.ac.uk/biostudies/studies/S-EPMC6513748biostudies-literatureSynthesis and evaluation of ⁶⁴Cu-radiolabeled NOTA-cetuximab (⁶⁴Cu-NOTA-C225) for immuno-PET imaging of EGFR expression.PMC6513748Yang XYang ZXia LGuo XHuang HZhu HXu XLiu TXie QLiu FLi NXin YfalseSynthesis and evaluation of ⁶⁴Cu-radiolabeled NOTA-cetuximab (⁶⁴Cu-NOTA-C225) for immuno-PET imaging of EGFR expression.<h4>Objective</h4>Epidermal growth factor receptor (EGFR) is overexpressed in a wide variety of solid tumors, serving as a well-characterized target for cancer imaging or therapy. In this study, we aimed to design and synthesize a radiotracer, ⁶⁴Cu-NOTA-C225, targeting EGFR for tumor positron emission tomography (PET) imaging.<h4>Methods</h4>Cetuximab (C225) was conjugated to a bifunctional chelator, p-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA), and further radiolabeled with copper-64 for PET imaging. ⁶⁴Cu-NOTA-IgG and Cy5.5-C225 were also synthesized as control probes. A431 and A549 mouse models were established for micro-PET and/or near-infrared fluorescence (NIRF) imaging.<h4>Results</h4>⁶⁴Cu-NOTA-C225 exhibited stability <i2019-01-01T00:00:00Z2019 Apr2026-06-01T06:32:31.43Z2026-04-08T10:01:33.95ZS-EPMC65137483115631010.21147/j.issn.1000-9604.2019.02.14