biostudies-literatureUnknown10Vamshi Krishna KIn microbial electrochemical systems, transport of electrons from bacteria to an electrode is the key to its functioning. However, the roles of several electron transport proteins, especially the membrane-bound dehydrogenases which link cellular metabolism to EET pathway are yet to be identified. NDH-2 is a non-proton pumping NADH dehydrogenase located in the inner membrane of several bacteria like <i>Bacillus subtilis</i>, <i>Escherichia coli</i>, etc. Unlike NADH dehydrogenase I, NDH-2 is not impeded by a high proton motive force thus helping in the increase of metabolic flux and carbon utilization. In the current study, NADH dehydrogenase II protein (NDH-2) was heterologously expressed from <i>B. subtilis</i> into <i>E. coli</i> BL21 (DE3) for enhancing electron flux through EET pathwayFrontiers in microbiology880https://www.ebi.ac.uk/biostudies/studies/S-EPMC6513898biostudies-literaturePurification and Characterization of NDH-2 Protein and Elucidating Its Role in Extracellular Electron Transport and Bioelectrogenic Activity.PMC6513898Vamshi Krishna KVenkata Mohan SfalsePurification and Characterization of NDH-2 Protein and Elucidating Its Role in Extracellular Electron Transport and Bioelectrogenic Activity.In microbial electrochemical systems, transport of electrons from bacteria to an electrode is the key to its functioning. However, the roles of several electron transport proteins, especially the membrane-bound dehydrogenases which link cellular metabolism to EET pathway are yet to be identified. NDH-2 is a non-proton pumping NADH dehydrogenase located in the inner membrane of several bacteria like <i>Bacillus subtilis</i>, <i>Escherichia coli</i>, etc. Unlike NADH dehydrogenase I, NDH-2 is not impeded by a high proton motive force thus helping in the increase of metabolic flux and carbon utilization. In the current study, NADH dehydrogenase II protein (NDH-2) was heterologously expressed from <i>B. subtilis</i> into <i>E. coli</i> BL21 (DE3) for enhancing electron flux through EET pathway2019-01-01T00:00:00Z20192026-04-08T05:34:36.512Z2019-06-06T23:14:18ZS-EPMC65138983113399610.3389/fmicb.2019.00880