biostudies-literatureAidukas TRCUK | Engineering and Physical Sciences Research CouncilEngineering and Physical Sciences Research Council7457https://www.ebi.ac.uk/biostudies/studies/S-EPMC6520337biostudies-literatureUnknown9(1)The revolution in low-cost consumer photography and computation provides fertile opportunity for a disruptive reduction in the cost of biomedical imaging. Conventional approaches to low-cost microscopy are fundamentally restricted, however, to modest field of view (FOV) and/or resolution. We report a low-cost microscopy technique, implemented with a Raspberry Pi single-board computer and color camera combined with Fourier ptychography (FP), to computationally construct 25-megapixel images with sub-micron resolution. New image-construction techniques were developed to enable the use of the low-cost Bayer color sensor, to compensate for the highly aberrated re-used camera lens and to compensate for misalignments associated with the 3D-printed microscope structure. This high ratio of performance to cost is of particular interest to high-throughput microscopy applications, ranging from drug discovery and digital pathology to health screening in low-income countries. 3D models and assembly instructions of our microscope are made available for open source use.Scientific reportsLow-cost, sub-micron resolution, wide-field computational microscopy using opensource hardware.PMC65203371807419EP/L016753/1Konda PCEckert RHarvey ARAidukas TWaller LfalseLow-cost, sub-micron resolution, wide-field computational microscopy using opensource hardware.The revolution in low-cost consumer photography and computation provides fertile opportunity for a disruptive reduction in the cost of biomedical imaging. Conventional approaches to low-cost microscopy are fundamentally restricted, however, to modest field of view (FOV) and/or resolution. We report a low-cost microscopy technique, implemented with a Raspberry Pi single-board computer and color camera combined with Fourier ptychography (FP), to computationally construct 25-megapixel images with sub-micron resolution. New image-construction techniques were developed to enable the use of the low-cost Bayer color sensor, to compensate for the highly aberrated re-used camera lens and to compensate for misalignments associated with the 3D-printed microscope structure. This high ratio of performance to cost is of particular interest to high-throughput microscopy applications, ranging from drug discovery and digital pathology to health screening in low-income countries. 3D models and assembly instructions of our microscope are made available for open source use.2019-01-01T00:00:00Z2019 May2026-05-06T21:53:20.27Z2019-06-06T23:15:53ZS-EPMC65203373109286710.1038/s41598-019-43845-9