{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(4)"],"submitter":["Auerswald H"],"funding":["European Research Council"],"pubmed_abstract":[": There are four distinct antigenic serotypes of dengue viruses (DENV-1-4). Sequential infections with different serotypes lead to crossreactive but also serotypespecific neutralizing antibody responses. Neutralization assays are considered as gold standard for serotype-specific antibody detection. However, for retrospective seroprevalence studies, access to large serum quantities is limited making neutralization assays well-nigh impossible. Therefore, a serological test, wasting only 10 µL serum, was developed using fusion proteins of maltose binding protein and E protein domain 3 (MBP-ED3) as antigens. Twelve MBP-ED3 antigens for DENV-1-4, three MBP-ED3 antigens for WNV, JEV, and TBEV, and MBP were dotted onto a single nitrocellulose strip. ED3 dot assay results were compared to virus ne"],"journal":["Viruses"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6521013"],"repository":["biostudies-literature"],"pubmed_title":["The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study."],"pmcid":["PMC6521013"],"funding_grant_id":["282 378"],"pubmed_authors":["Franzke K","Auerswald H","Schreiber S","Duong V","Hulsemann J","Buchy P","Schreiber M","Klepsch L","Y B","Kann S"],"additional_accession":[]},"is_claimable":false,"name":"The Dengue ED3 Dot Assay, a Novel Serological Test for the Detection of Denguevirus Type-Specific Antibodies and Its Application in a Retrospective Seroprevalence Study.","description":": There are four distinct antigenic serotypes of dengue viruses (DENV-1-4). Sequential infections with different serotypes lead to crossreactive but also serotypespecific neutralizing antibody responses. Neutralization assays are considered as gold standard for serotype-specific antibody detection. However, for retrospective seroprevalence studies, access to large serum quantities is limited making neutralization assays well-nigh impossible. Therefore, a serological test, wasting only 10 µL serum, was developed using fusion proteins of maltose binding protein and E protein domain 3 (MBP-ED3) as antigens. Twelve MBP-ED3 antigens for DENV-1-4, three MBP-ED3 antigens for WNV, JEV, and TBEV, and MBP were dotted onto a single nitrocellulose strip. ED3 dot assay results were compared to virus ne","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Mar","modification":"2025-04-05T20:26:40.375Z","creation":"2019-07-01T13:52:27Z"},"accession":"S-EPMC6521013","cross_references":{"pubmed":["30934772"],"doi":["10.3390/v11040304"]}}