{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suzuki T"],"funding":["Japan Society for the Promotion of Science (JSPS) KAKENHI grant"],"pagination":["E344"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6521110"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(4)"],"pubmed_abstract":["To examine the role of RNA silencing in plant defenses against viroids, a Dicer-like 2 and 4 (DCL2&4)-double knockdown transgenic tomato plant line, 72E, was created. The expression of endogenous <i>SlDCL2</i>s and <i>SlDCL4</i> in line 72E decreased to about a half that of the empty cassette line, EC. When challenged with potato spindle tuber viroid (PSTVd), line 72E showed significantly higher levels of PSTVd accumulation early in the course of the infection and lethal systemic necrosis late in the infection. The size distribution of PSTVd-derived small RNAs was significantly different with the number of RNAs of 21 and 22 nucleotides (nt) in line 72E, at approximately 66.7% and 5% of those in line EC, respectively. Conversely, the numbers of 24 nt species increased by 1100%. Furthermore,"],"journal":["Viruses"],"pubmed_title":["RNAi-Mediated Down-Regulation of Dicer-Like 2 and 4 Changes the Response of 'Moneymaker' Tomato to Potato Spindle Tuber Viroid Infection from Tolerance to Lethal Systemic Necrosis, Accompanied by Up-Regulation of miR398, 398a-3p and Production of Excessive Amount of Reactive Oxygen Species."],"pmcid":["PMC6521110"],"funding_grant_id":["15H04455 and 18H0221"],"pubmed_authors":["Taneda A","Maeda H","Suzuki T","Ikeda S","Sugawara K","Okuta M","Fujibayashi M","Kasai A","Sano T"],"additional_accession":[]},"is_claimable":false,"name":"RNAi-Mediated Down-Regulation of Dicer-Like 2 and 4 Changes the Response of 'Moneymaker' Tomato to Potato Spindle Tuber Viroid Infection from Tolerance to Lethal Systemic Necrosis, Accompanied by Up-Regulation of miR398, 398a-3p and Production of Excessive Amount of Reactive Oxygen Species.","description":"To examine the role of RNA silencing in plant defenses against viroids, a Dicer-like 2 and 4 (DCL2&4)-double knockdown transgenic tomato plant line, 72E, was created. The expression of endogenous <i>SlDCL2</i>s and <i>SlDCL4</i> in line 72E decreased to about a half that of the empty cassette line, EC. When challenged with potato spindle tuber viroid (PSTVd), line 72E showed significantly higher levels of PSTVd accumulation early in the course of the infection and lethal systemic necrosis late in the infection. The size distribution of PSTVd-derived small RNAs was significantly different with the number of RNAs of 21 and 22 nucleotides (nt) in line 72E, at approximately 66.7% and 5% of those in line EC, respectively. Conversely, the numbers of 24 nt species increased by 1100%. Furthermore,","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Apr","modification":"2026-04-08T05:24:10.725Z","creation":"2019-07-01T13:52:31Z"},"accession":"S-EPMC6521110","cross_references":{"pubmed":["31013904"],"doi":["10.3390/v11040344"]}}