biostudies-literature00530Georas SNIAID NIH HHSNIEHS NIH HHSNHLBI NIH HHSParkes Family FoundationNIH1257-1265https://www.ebi.ac.uk/biostudies/studies/S-EPMC6522330biostudies-literatureUnknown56(12)<i>Objective</i>: Airway epithelial barrier dysfunction is emerging as an important feature of asthma pathogenesis, but this is difficult to measure in individual subjects. We aimed to develop a noninvasive way to measure airway permeability in asthma. <i>Methods</i>: Healthy controls and subjects with mild asthma inhaled dry powder mannitol in a dose-escalating manner on two separate occasions, stopping at 155 mg or 315 mg. Serum mannitol levels were measured at baseline and then 30, 90, and 150 min after mannitol inhalation. Mannitol absorption was compared with measurements of airflow obstruction (FEV1) and airway inflammation (FeNO). <i>Results</i>: Serum mannitol levels increased in a time- and dose-dependent manner in both healthy control and subjects with asthma. There were no significant differences in mannitol absorption when comparing healthy controls and subjects with asthma. Mannitol absorption did not correlate with markers of airway obstruction or inflammation. <i>Conclusions</i>: Measuring serum concentrations of mannitol after inhalation challenge can potentially provide insights into airway barrier function in asthma.The Journal of asthma : official journal of the Association for the Care of AsthmaThe leaky lung test: a pilot study using inhaled mannitol to measure airway barrier function in asthma.PMC6522330F32 HL110718T32 HL066988R01 HL014224T32 AI007285T32 ES007026P30 ES001247F31 HL140795T32 HL140795R01 HL122424Le KGeoras SVeazey JSmyth TMeddings JHillman SRansom NEliseeva S53falseThe leaky lung test: a pilot study using inhaled mannitol to measure airway barrier function in asthma.<i>Objective</i>: Airway epithelial barrier dysfunction is emerging as an important feature of asthma pathogenesis, but this is difficult to measure in individual subjects. We aimed to develop a noninvasive way to measure airway permeability in asthma. <i>Methods</i>: Healthy controls and subjects with mild asthma inhaled dry powder mannitol in a dose-escalating manner on two separate occasions, stopping at 155 mg or 315 mg. Serum mannitol levels were measured at baseline and then 30, 90, and 150 min after mannitol inhalation. Mannitol absorption was compared with measurements of airflow obstruction (FEV1) and airway inflammation (FeNO). <i>Results</i>: Serum mannitol levels increased in a time- and dose-dependent manner in both healthy control and subjects with asthma. There were no significant differences in mannitol absorption when comparing healthy controls and subjects with asthma. Mannitol absorption did not correlate with markers of airway obstruction or inflammation. <i>Conclusions</i>: Measuring serum concentrations of mannitol after inhalation challenge can potentially provide insights into airway barrier function in asthma.2019-01-01T00:00:00Z2019 Dec2024-11-09T10:23:44.669Z2021-02-20T02:51:30ZS-EPMC65223303044414310.1080/02770903.2018.1536145