{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liebisch G"],"funding":["Deutsche Forschungsgemeinschaft","Seventh Framework Programme"],"pagination":["E121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6523859"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(4)"],"pubmed_abstract":["Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) by the gut microbiota relevant for microbe⁻host communication. Here, we present a method for the quantification of SCFAs in fecal samples by liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The method includes acetate, propionate, butyrate, and isobutyrate with a run time of 4 min. The reproducible (coefficients of variation (CV) below 10%) quantification of SCFAs in human fecal samples was achieved by the application of stable isotope labelled internal standards. The specificity was demonstrated by the introduction of a quantifier and qualifier ions. The method was applied to investigate the pre-an"],"journal":["Biomolecules"],"pubmed_title":["Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry-Investigation of Pre-Analytic Stability."],"pmcid":["PMC6523859"],"funding_grant_id":["EC453/2-1","LI 923/4-1","613979"],"pubmed_authors":["Yoon H","Ottl V","Ecker J","Matysik S","Haller D","Liebisch G","Schweizer S","Schott HF","Roth S","Holler E","Burkhardt R"],"additional_accession":[]},"is_claimable":false,"name":"Quantification of Fecal Short Chain Fatty Acids by Liquid Chromatography Tandem Mass Spectrometry-Investigation of Pre-Analytic Stability.","description":"Short chain fatty acids (SCFAs) are generated by the degradation and fermentation of complex carbohydrates, (i.e., dietary fiber) by the gut microbiota relevant for microbe⁻host communication. Here, we present a method for the quantification of SCFAs in fecal samples by liquid chromatography tandem mass spectrometry (LC-MS/MS) upon derivatization to 3-nitrophenylhydrazones (3NPH). The method includes acetate, propionate, butyrate, and isobutyrate with a run time of 4 min. The reproducible (coefficients of variation (CV) below 10%) quantification of SCFAs in human fecal samples was achieved by the application of stable isotope labelled internal standards. The specificity was demonstrated by the introduction of a quantifier and qualifier ions. The method was applied to investigate the pre-an","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Mar","modification":"2026-05-06T22:39:06.265Z","creation":"2019-07-01T13:53:13Z"},"accession":"S-EPMC6523859","cross_references":{"pubmed":["30925749"],"doi":["10.3390/biom9040121"]}}