biostudies-literatureMalkocs TMinistry of Human Capacitiese01245https://www.ebi.ac.uk/biostudies/studies/S-EPMC6526657biostudies-literatureUnknown7(5)<h4>Premise</h4><i>Gladiolus palustris</i> (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species.<h4>Methods and results</h4>We utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite-enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62-3.08 alleles per population (effective alleles: 1.58-2.08) that indicated moderate to high genetic diversity values (0.28-0.44) in three pilot populations. Cross-species amplification was less effective in <i>G. imbricatus</i> and <i>G. tenuis</i>.<h4>Conclusions</h4>The primers reported here can be used for the population genetic characterization of <i>G. palustris</i>. They will help us to better understand the conservation genetics of this highly endangered species.Applications in plant sciencesIsolation and characterization of 15 SSR loci for the endangered European tetraploid species <i>Gladiolus palustris</i> (Iridaceae).PMC6526657NTP-NFTÖ-18-B-0279Meglecz ECservenka JBereczki JSramko GAlmerekova SMalkocs TfalseIsolation and characterization of 15 SSR loci for the endangered European tetraploid species <i>Gladiolus palustris</i> (Iridaceae).<h4>Premise</h4><i>Gladiolus palustris</i> (Iridaceae) is an endangered European perennial tetraploid herb with special conservation interest in the European Union. Microsatellite markers can serve as effective tools for the conservation genetics of this species.<h4>Methods and results</h4>We utilized a 454 pyrosequencing approach to identify simple sequence repeat (SSR) regions in a microsatellite-enriched library. Of all SSR regions, 46 were screened for specific PCR amplification, and 15 were found to be applicable in the target species. We found 1.62-3.08 alleles per population (effective alleles: 1.58-2.08) that indicated moderate to high genetic diversity values (0.28-0.44) in three pilot populations. Cross-species amplification was less effective in <i>G. imbricatus</i> and <i>G. tenuis</i>.<h4>Conclusions</h4>The primers reported here can be used for the population genetic characterization of <i>G. palustris</i>. They will help us to better understand the conservation genetics of this highly endangered species.2019-01-01T00:00:00Z2019 May2026-05-07T14:50:09.6Z2019-06-06T23:17:34ZS-EPMC65266573113951110.1002/aps3.1245