biostudies-literatureSchmidt MENIAID NIH HHSNCI NIH HHSNIGMS NIH HHS3234-3245https://www.ebi.ac.uk/biostudies/studies/S-EPMC6529240biostudies-literatureUnknown202(11)Respiratory syncytial virus (RSV) is the leading cause of severe respiratory tract infection in infants and young children, but no vaccine is currently available. Live-attenuated vaccines represent an attractive immunization approach; however, balancing attenuation while retaining sufficient immunogenicity and efficacy has prevented the successful development of such a vaccine. Recently, a recombinant RSV strain lacking the gene that encodes the matrix (M) protein (RSV M-null) was developed. The M protein is required for virion assembly following infection of a host cell but is not necessary for either genome replication or gene expression. Therefore, infection with RSV M-null produces all viral proteins except M but does not generate infectious virus progeny, resulting in a single-cycle iJournal of immunology (Baltimore, Md. : 1950)Vaccination with a Single-Cycle Respiratory Syncytial Virus Is Immunogenic and Protective in Mice.PMC6529240P20 GM103648R01 AI124093T32 AI007485P30 CA086862Oomens AGPVarga SMSchmidt MEfalseVaccination with a Single-Cycle Respiratory Syncytial Virus Is Immunogenic and Protective in Mice.Respiratory syncytial virus (RSV) is the leading cause of severe respiratory tract infection in infants and young children, but no vaccine is currently available. Live-attenuated vaccines represent an attractive immunization approach; however, balancing attenuation while retaining sufficient immunogenicity and efficacy has prevented the successful development of such a vaccine. Recently, a recombinant RSV strain lacking the gene that encodes the matrix (M) protein (RSV M-null) was developed. The M protein is required for virion assembly following infection of a host cell but is not necessary for either genome replication or gene expression. Therefore, infection with RSV M-null produces all viral proteins except M but does not generate infectious virus progeny, resulting in a single-cycle i2019-01-01T00:00:00Z2019 Jun2025-04-04T14:00:25.157Z2020-06-04T07:05:09ZS-EPMC65292403100401010.4049/jimmunol.1900050