biostudies-literatureUnknown5(1)Tahrioui ABiofilms are structured microbial communities that are the leading cause of numerous chronic infections which are difficult to eradicate. Within the lungs of individuals with cystic fibrosis (CF), <i>Pseudomonas aeruginosa</i> causes persistent biofilm infection that is commonly treated with aminoglycoside antibiotics such as tobramycin. However, sublethal concentrations of this aminoglycoside were previously shown to increase biofilm formation by <i>P. aeruginosa</i>, but the underlying adaptive mechanisms still remain elusive. Herein, we combined confocal laser scanning microscope analyses, proteomics profiling, gene expression assays and phenotypic studies to unravel <i>P. aeruginosa</i> potential adaptive mechanisms in response to tobramycin exposure during biofilm growth. Under this cNPJ biofilms and microbiomes15https://www.ebi.ac.uk/biostudies/studies/S-EPMC6533273biostudies-literatureExtracellular DNA release, quorum sensing, and PrrF1/F2 small RNAs are key players in <i>Pseudomonas aeruginosa</i> tobramycin-enhanced biofilm formation.PMC6533273Rodrigues SBouffartigues EGroleau MCLesouhaitier OBrenner-Weiss GFeuilloley MDuchesne RTortuel DMaillot ODufour AChevalier STahrioui AHardouin JDeziel ETaupin LOrange NCornelis PfalseExtracellular DNA release, quorum sensing, and PrrF1/F2 small RNAs are key players in <i>Pseudomonas aeruginosa</i> tobramycin-enhanced biofilm formation.Biofilms are structured microbial communities that are the leading cause of numerous chronic infections which are difficult to eradicate. Within the lungs of individuals with cystic fibrosis (CF), <i>Pseudomonas aeruginosa</i> causes persistent biofilm infection that is commonly treated with aminoglycoside antibiotics such as tobramycin. However, sublethal concentrations of this aminoglycoside were previously shown to increase biofilm formation by <i>P. aeruginosa</i>, but the underlying adaptive mechanisms still remain elusive. Herein, we combined confocal laser scanning microscope analyses, proteomics profiling, gene expression assays and phenotypic studies to unravel <i>P. aeruginosa</i> potential adaptive mechanisms in response to tobramycin exposure during biofilm growth. Under this c2019-01-01T00:00:00Z20192025-04-04T21:40:24.554Z2019-07-01T13:54:51ZS-EPMC65332733114934510.1038/s41522-019-0088-3