biostudies-literatureUnknown175(3)de Groot AFAmgenDutch Cancer SocietySanofiNovartis<h4>Purpose</h4>Tumor-infiltrating lymphocytes (TILs) are associated with pathological complete response (pCR) and survival after neoadjuvant chemotherapy (NAC) in patients with early breast cancer. We investigated the prognostic and predictive role of TILs, macrophages, and HLA class 1 expression after NAC with or without the potentially immune modulating compound zoledronic acid (ZA).<h4>Methods</h4>Baseline tumor biopsies from 196 patients in the NEOZOTAC trial were analyzed for CD8 (cytotoxic T-cells), FoxP3 (regulatory T-cells), CD68 (macrophages), and HLA class I (HCA2/HC10) expression by immunohistochemistry and subsequently related to pCR and disease-free survival (DFS).<h4>Results</h4>A strong intratumoral CD8+ infiltration or expression of HLA class 1 by cancer cells was associatBreast cancer research and treatment605-615https://www.ebi.ac.uk/biostudies/studies/S-EPMC6534526biostudies-literatureStrong CD8+ lymphocyte infiltration in combination with expression of HLA class I is associated with better tumor control in breast cancer patients treated with neoadjuvant chemotherapy.PMC6534526Putter HSmit VTHBMEngels CCde Groot AFBlok EJvan de Velde CJHMeershoek-Klein Kranenbarg Evan der Burg SHCharehbili ADekker-Ensink NGKuppen PJKLiefers GJNortier JWRKroep JRfalseStrong CD8+ lymphocyte infiltration in combination with expression of HLA class I is associated with better tumor control in breast cancer patients treated with neoadjuvant chemotherapy.<h4>Purpose</h4>Tumor-infiltrating lymphocytes (TILs) are associated with pathological complete response (pCR) and survival after neoadjuvant chemotherapy (NAC) in patients with early breast cancer. We investigated the prognostic and predictive role of TILs, macrophages, and HLA class 1 expression after NAC with or without the potentially immune modulating compound zoledronic acid (ZA).<h4>Methods</h4>Baseline tumor biopsies from 196 patients in the NEOZOTAC trial were analyzed for CD8 (cytotoxic T-cells), FoxP3 (regulatory T-cells), CD68 (macrophages), and HLA class I (HCA2/HC10) expression by immunohistochemistry and subsequently related to pCR and disease-free survival (DFS).<h4>Results</h4>A strong intratumoral CD8+ infiltration or expression of HLA class 1 by cancer cells was associat2019-01-01T00:00:00Z2019 Jun2025-05-18T11:02:39.795Z2025-05-18T11:02:39.795ZS-EPMC65345263086839210.1007/s10549-019-05195-y