{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Merz SF"],"pubmed_abstract":["Cardioprotection by salvage of the infarct-affected myocardium is an unmet yet highly desired therapeutic goal. To develop new dedicated therapies, experimental myocardial ischemia/reperfusion (I/R) injury would require methods to simultaneously characterize extent and localization of the damage and the ensuing inflammatory responses in whole hearts over time. Here we present a three-dimensional (3D), simultaneous quantitative investigation of key I/R injury-components by combining bleaching-augmented solvent-based non-toxic clearing (BALANCE) using ethyl cinnamate (ECi) with light sheet fluorescence microscopy. This allows structural analyses of fluorescence-labeled I/R hearts with exceptional detail. We discover and 3D-quantify distinguishable acute and late vascular I/R damage zones. Th"],"journal":["Nature communications"],"pagination":["2312"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6534576"],"repository":["biostudies-literature"],"pubmed_title":["Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response."],"pmcid":["PMC6534576"],"pubmed_authors":["Detzer J","Squire A","Merz SF","Stock P","Hendgen-Cotta UB","Engel DR","Soun C","Totzeck M","Klode J","Lorchner H","Bornemann L","Rassaf T","Michel L","Korste S","Kamler M","Hermann DM","Gunzer M"],"additional_accession":[]},"is_claimable":false,"name":"Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response.","description":"Cardioprotection by salvage of the infarct-affected myocardium is an unmet yet highly desired therapeutic goal. To develop new dedicated therapies, experimental myocardial ischemia/reperfusion (I/R) injury would require methods to simultaneously characterize extent and localization of the damage and the ensuing inflammatory responses in whole hearts over time. Here we present a three-dimensional (3D), simultaneous quantitative investigation of key I/R injury-components by combining bleaching-augmented solvent-based non-toxic clearing (BALANCE) using ethyl cinnamate (ECi) with light sheet fluorescence microscopy. This allows structural analyses of fluorescence-labeled I/R hearts with exceptional detail. We discover and 3D-quantify distinguishable acute and late vascular I/R damage zones. Th","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2026-05-07T12:21:05.293Z","creation":"2026-04-07T22:55:00.416Z"},"accession":"S-EPMC6534576","cross_references":{"pubmed":["31127113"],"doi":["10.1038/s41467-019-10338-2"]}}