biostudies-literatureDjinni IAlgerian Ministry of Higher Education and Scientific Researche01695https://www.ebi.ac.uk/biostudies/studies/S-EPMC6538969biostudies-literatureUnknown5(5)The novel bioactive actinobacterial strain GSBNT10 obtained from a Saharan soil, was taxonomically characterized using a polyphasic approach. 16S rRNA gene sequence analysis supported the classification of the isolate within the genus <i>Streptomyces</i> indicating it as a novel species. The major metabolite responsible of the bioactivity was purified and structurally characterized as actinomycin D (act-D) by mass spectrometric and nuclear magnetic resonance analyses Plackett-Burman design (PBD) and response surface methodology (RSM) were applied in order to optimize the medium formulation for the production of this bioactive metabolite. By PBD experiments, NaNO₃, K₂HPO₄ and initial pH value were selected as significant variables affecting the metabolite production. Central Composite Design (CCD) showed that adjustment of the fermentative medium at pH 8.25, K₂HPO₄ at 0.2 gL^-1 and NaNO₃ at 3.76 gL^-1 were the values suiting the production of act-D. Moreover, the results obtained by the statistical approach were confirmed by act-D detection using the HPLC equipped with a diode array detector and coupled online with electrospray-mass spectrometry (ESIMS) technique. act-D production was highly stimulated, obtaining a good yield (656.46 mgL^-1) which corresponds to a 58.56% increase compared with the non-optimized conditions and data from LC-ESIMS technique efficiently confirmed the forecast from RSM.HeliyonModeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel <i>Streptomyces</i> sp. strain from a Saharan soil.PMC6538969F00620140028Chaabane Chaouch FSouagui SDjoudi WMancini IDjinni IDefant AKecha MfalseModeling improved production of the chemotherapeutic polypeptide actinomycin D by a novel <i>Streptomyces</i> sp. strain from a Saharan soil.The novel bioactive actinobacterial strain GSBNT10 obtained from a Saharan soil, was taxonomically characterized using a polyphasic approach. 16S rRNA gene sequence analysis supported the classification of the isolate within the genus <i>Streptomyces</i> indicating it as a novel species. The major metabolite responsible of the bioactivity was purified and structurally characterized as actinomycin D (act-D) by mass spectrometric and nuclear magnetic resonance analyses Plackett-Burman design (PBD) and response surface methodology (RSM) were applied in order to optimize the medium formulation for the production of this bioactive metabolite. By PBD experiments, NaNO₃, K₂HPO₄ and initial pH value were selected as significant variables affecting the metabolite production. Central Composite Design (CCD) showed that adjustment of the fermentative medium at pH 8.25, K₂HPO₄ at 0.2 gL^-1 and NaNO₃ at 3.76 gL^-1 were the values suiting the production of act-D. Moreover, the results obtained by the statistical approach were confirmed by act-D detection using the HPLC equipped with a diode array detector and coupled online with electrospray-mass spectrometry (ESIMS) technique. act-D production was highly stimulated, obtaining a good yield (656.46 mgL^-1) which corresponds to a 58.56% increase compared with the non-optimized conditions and data from LC-ESIMS technique efficiently confirmed the forecast from RSM.2019-01-01T00:00:00Z2019 May2024-12-04T04:43:16.746Z2019-07-01T13:56:16ZS-EPMC65389693119370210.1016/j.heliyon.2019.e01695