{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rybakovsky E"],"funding":["NIAID NIH HHS"],"pagination":["99-114"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6542284"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Epithelial barrier function studies often attribute alterations in barrier function to induced changes in tight junctional (TJ) complexes. The occurrence of spontaneous and cytokine-induced, focal cell detachment in cell layers of the human gingival epithelial cell line, Gie-3B11, highlights the danger of this assumption without confirmatory experimentation. Gie-3B11 cell layers manifest morphological polarity, TJ complexes and barrier function after confluence but fail to then maintain a stable epithelial barrier. Transepithelial electrical resistance rises to over 100 ohms x cm<sup>2</sup> a few days after seeding cell layers at a confluent density, but then spontaneously declines, with simultaneous, inverse changes in transepithelial <sup>14</sup>C-D-mannitol diffusion rates. This barri"],"journal":["Trends in cell & molecular biology"],"pubmed_title":["Spontaneous and cytokine-induced hole formation in epithelial cell layers: Implications for barrier function studies with the gingival cell culture, Gie-3B11, and other epithelial models."],"pmcid":["PMC6542284"],"funding_grant_id":["R21 AI139392","R21 AI059277"],"pubmed_authors":["DiGuilio KM","Hoxha K","Mullin JM","Rybakovsky E","Thomas S","McCluskey ES","Friday CL","Callaghan PJ","Moskalenko DV","Buleza NB","Zuo B"],"additional_accession":[]},"is_claimable":false,"name":"Spontaneous and cytokine-induced hole formation in epithelial cell layers: Implications for barrier function studies with the gingival cell culture, Gie-3B11, and other epithelial models.","description":"Epithelial barrier function studies often attribute alterations in barrier function to induced changes in tight junctional (TJ) complexes. The occurrence of spontaneous and cytokine-induced, focal cell detachment in cell layers of the human gingival epithelial cell line, Gie-3B11, highlights the danger of this assumption without confirmatory experimentation. Gie-3B11 cell layers manifest morphological polarity, TJ complexes and barrier function after confluence but fail to then maintain a stable epithelial barrier. Transepithelial electrical resistance rises to over 100 ohms x cm<sup>2</sup> a few days after seeding cell layers at a confluent density, but then spontaneously declines, with simultaneous, inverse changes in transepithelial <sup>14</sup>C-D-mannitol diffusion rates. This barri","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2025-04-04T02:39:51.13Z","creation":"2019-07-01T13:56:54Z"},"accession":"S-EPMC6542284","cross_references":{"pubmed":["31156296"]}}