biostudies-literatureRybakovsky ENIAID NIH HHS99-114https://www.ebi.ac.uk/biostudies/studies/S-EPMC6542284biostudies-literatureUnknown13Epithelial barrier function studies often attribute alterations in barrier function to induced changes in tight junctional (TJ) complexes. The occurrence of spontaneous and cytokine-induced, focal cell detachment in cell layers of the human gingival epithelial cell line, Gie-3B11, highlights the danger of this assumption without confirmatory experimentation. Gie-3B11 cell layers manifest morphological polarity, TJ complexes and barrier function after confluence but fail to then maintain a stable epithelial barrier. Transepithelial electrical resistance rises to over 100 ohms x cm² a few days after seeding cell layers at a confluent density, but then spontaneously declines, with simultaneous, inverse changes in transepithelial ¹⁴C-D-mannitol diffusion rates. This barriTrends in cell & molecular biologySpontaneous and cytokine-induced hole formation in epithelial cell layers: Implications for barrier function studies with the gingival cell culture, Gie-3B11, and other epithelial models.PMC6542284R21 AI139392R21 AI059277DiGuilio KMHoxha KMullin JMRybakovsky EThomas SMcCluskey ESFriday CLCallaghan PJMoskalenko DVBuleza NBZuo BfalseSpontaneous and cytokine-induced hole formation in epithelial cell layers: Implications for barrier function studies with the gingival cell culture, Gie-3B11, and other epithelial models.Epithelial barrier function studies often attribute alterations in barrier function to induced changes in tight junctional (TJ) complexes. The occurrence of spontaneous and cytokine-induced, focal cell detachment in cell layers of the human gingival epithelial cell line, Gie-3B11, highlights the danger of this assumption without confirmatory experimentation. Gie-3B11 cell layers manifest morphological polarity, TJ complexes and barrier function after confluence but fail to then maintain a stable epithelial barrier. Transepithelial electrical resistance rises to over 100 ohms x cm² a few days after seeding cell layers at a confluent density, but then spontaneously declines, with simultaneous, inverse changes in transepithelial ¹⁴C-D-mannitol diffusion rates. This barri2018-01-01T00:00:00Z20182025-04-04T02:39:51.13Z2019-07-01T13:56:54ZS-EPMC654228431156296