biostudies-literaturePfeifer MNational Institute for Health Research (NIHR)Marie Curie3423-3433https://www.ebi.ac.uk/biostudies/studies/S-EPMC6545462biostudies-literatureUnknown202(12)Induction of programmed DNA damage and its recognition and repair are fundamental for B cell development. The ssDNA-binding protein SSB1 has been described in human cells as essential for the recognition and repair of DNA damage. To study its relevance for B cells, we recently developed <i>Ssb1</i> ^-/- and conditional <i>Ssb1</i> ^-/- mice. Although SSB1 loss did not affect B cell development, <i>Ssb1</i> ^-/- cells exhibited compensatory expression of its homolog SSB2. We have now generated <i>Ssb2</i> ^-/- mice and show in this study that SSB2 is also dispensable for B cell development and DNA damage response activation. In contrast to the single loss of <i>Ssb1</i> or <i>Ssb2</i>, however, combined SSB1/2 deficiency caused a defect in early B celJournal of immunology (Baltimore, Md. : 1950)SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.PMC6545462NIHR-RP-011-053P49318Robinson MEBoulianne BLippert TPHo HNFeldhahn NStebbing JPfeifer MBrem RfalseSSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.Induction of programmed DNA damage and its recognition and repair are fundamental for B cell development. The ssDNA-binding protein SSB1 has been described in human cells as essential for the recognition and repair of DNA damage. To study its relevance for B cells, we recently developed <i>Ssb1</i> ^-/- and conditional <i>Ssb1</i> ^-/- mice. Although SSB1 loss did not affect B cell development, <i>Ssb1</i> ^-/- cells exhibited compensatory expression of its homolog SSB2. We have now generated <i>Ssb2</i> ^-/- mice and show in this study that SSB2 is also dispensable for B cell development and DNA damage response activation. In contrast to the single loss of <i>Ssb1</i> or <i>Ssb2</i>, however, combined SSB1/2 deficiency caused a defect in early B cel2019-01-01T00:00:00Z2019 Jun2025-04-18T14:12:10.53Z2019-07-24T07:08:11ZS-EPMC65454623108559110.4049/jimmunol.1801618