biostudies-literatureAl Hinai EAGovernment of the Sultanate of OmanBiotechnology and Biological Sciences Research Council398-411https://www.ebi.ac.uk/biostudies/studies/S-EPMC6546321biostudies-literatureUnknown10(3)<b>Background</b>: A greater understanding of mechanisms explaining the interactions between diet and the gut microbiota in colorectal cancer is desirable. Genotoxic microbial metabolites present in the colon may be implicated in carcinogenesis and potentially influenced by diet. <b>Aims</b>: We hypothesised that microbial p-cresol is a colonic genotoxin and set out to model potential exposures in the colon and the effects of these exposures on colonic cells. <b>Methods</b>: Batch culture fermentations with human faecal inoculate were used to determine the synthesis of p-cresol and other metabolites in response to various substrates. The fermentation supernatants were evaluated for genotoxicity and the independent effects of p-cresol on colonic cells were studied <i>in vitro</i>. <b>ResultGut microbesModelling the role of microbial p-cresol in colorectal genotoxicity.PMC6546321BB/M006484/1Commane DMRowland IRAl Hinai EASwann JWalton GEKullamethee PfalseModelling the role of microbial p-cresol in colorectal genotoxicity.<b>Background</b>: A greater understanding of mechanisms explaining the interactions between diet and the gut microbiota in colorectal cancer is desirable. Genotoxic microbial metabolites present in the colon may be implicated in carcinogenesis and potentially influenced by diet. <b>Aims</b>: We hypothesised that microbial p-cresol is a colonic genotoxin and set out to model potential exposures in the colon and the effects of these exposures on colonic cells. <b>Methods</b>: Batch culture fermentations with human faecal inoculate were used to determine the synthesis of p-cresol and other metabolites in response to various substrates. The fermentation supernatants were evaluated for genotoxicity and the independent effects of p-cresol on colonic cells were studied <i>in vitro</i>. <b>Result2019-01-01T00:00:00Z20192025-05-18T13:16:19.622Z2025-05-18T13:16:19.622ZS-EPMC65463213035955310.1080/19490976.2018.1534514