{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Porsch M"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["661-674"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6546408"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(5)"],"pubmed_abstract":["Acquired tamoxifen resistance is a persistent problem for the treatment of estrogen receptor positive, premenopausal breast cancer patients and predictive biomarkers are still elusive. We here analyzed gene expression changes in a cellular model to identify early and late changes upon tamoxifen exposure and thereby novel prognostic biomarkers. Estrogen receptor positive MCF-7 cells were incubated with 4OH-tamoxifen (10 nM) and gene expression analyzed by array hybridization during 12 weeks. Array results were confirmed by nCounter- and qRT-PCR technique. Pathway enrichment analysis revealed that early responses concerned mainly amine synthesis and NRF2-related signaling and evolved into a stable gene expression pattern within 4 weeks characterized by changes in glucuronidation-, estrogen m"],"journal":["RNA biology"],"pubmed_title":["Time resolved gene expression analysis during tamoxifen adaption of MCF-7 cells identifies long non-coding RNAs with prognostic impact."],"pmcid":["PMC6546408"],"funding_grant_id":["KA2663/3-1","FZT118"],"pubmed_authors":["Kalinski T","Haybaeck J","Hoffmann K","Grosse I","Nass N","Ozdemir E","Wisniewski M","Bull F","Ignatov A","Porsch M","Graf S"],"additional_accession":[]},"is_claimable":false,"name":"Time resolved gene expression analysis during tamoxifen adaption of MCF-7 cells identifies long non-coding RNAs with prognostic impact.","description":"Acquired tamoxifen resistance is a persistent problem for the treatment of estrogen receptor positive, premenopausal breast cancer patients and predictive biomarkers are still elusive. We here analyzed gene expression changes in a cellular model to identify early and late changes upon tamoxifen exposure and thereby novel prognostic biomarkers. Estrogen receptor positive MCF-7 cells were incubated with 4OH-tamoxifen (10 nM) and gene expression analyzed by array hybridization during 12 weeks. Array results were confirmed by nCounter- and qRT-PCR technique. Pathway enrichment analysis revealed that early responses concerned mainly amine synthesis and NRF2-related signaling and evolved into a stable gene expression pattern within 4 weeks characterized by changes in glucuronidation-, estrogen m","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2026-04-08T02:12:38.201Z","creation":"2025-05-18T13:33:03.27Z"},"accession":"S-EPMC6546408","cross_references":{"pubmed":["30760083"],"doi":["10.1080/15476286.2019.1581597"]}}