{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang TP"],"funding":["NIBIB NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","Howard Hughes Medical Institute","NIAID NIH HHS","NHGRI NIH HHS","NIGMS NIH HHS","Division of Intramural Research, National Institute of Allergy and Infectious Diseases","U.S. Department of Health & Human Services | NIH | National Institute of Biomedical Imaging and Bioengineering"],"pagination":["626-631"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6551276"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(6)"],"pubmed_abstract":["Base editing requires that the target sequence satisfy the protospacer adjacent motif requirement of the Cas9 domain and that the target nucleotide be located within the editing window of the base editor. To increase the targeting scope of base editors, we engineered six optimized adenine base editors (ABEmax variants) that use SpCas9 variants compatible with non-NGG protospacer adjacent motifs. To increase the range of target bases that can be modified within the protospacer, we use circularly permuted Cas9 variants to produce four cytosine and four adenine base editors with an editing window expanded from ~4-5 nucleotides to up to ~8-9 nucleotides and reduced byproduct formation. This set of base editors improves the targeting scope of cytosine and adenine base editing."],"journal":["Nature biotechnology"],"pubmed_title":["Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors."],"pmcid":["PMC6551276"],"funding_grant_id":["R35 GM118062","T32 GM095450","U01 AI142756","DP2 EB018658","R00 GM118909","K99 GM118909","R01 EB022376","R01 GM127463","RM1 HG009490"],"pubmed_authors":["Oakes BL","Miller SM","Gaudelli NM","Fellmann C","Savage DF","Liu DR","Huang TP","Zhao KT"],"additional_accession":[]},"is_claimable":false,"name":"Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.","description":"Base editing requires that the target sequence satisfy the protospacer adjacent motif requirement of the Cas9 domain and that the target nucleotide be located within the editing window of the base editor. To increase the targeting scope of base editors, we engineered six optimized adenine base editors (ABEmax variants) that use SpCas9 variants compatible with non-NGG protospacer adjacent motifs. To increase the range of target bases that can be modified within the protospacer, we use circularly permuted Cas9 variants to produce four cytosine and four adenine base editors with an editing window expanded from ~4-5 nucleotides to up to ~8-9 nucleotides and reduced byproduct formation. This set of base editors improves the targeting scope of cytosine and adenine base editing.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2024-11-13T12:47:00.654Z","creation":"2020-05-21T20:03:39Z"},"accession":"S-EPMC6551276","cross_references":{"pubmed":["31110355"],"doi":["10.1038/s41587-019-0134-y"]}}