biostudies-literatureMehta HHNIA NIH HHSU.S. Department of DefenseNational Institute on Aging88https://www.ebi.ac.uk/biostudies/studies/S-EPMC6554247biostudies-literatureUnknown15(6)<h4>Introduction</h4>The mitochondrial-derived peptides (MDPs) are a novel group of natural occurring peptides that have important signaling functions and biological activity. Both humanin and small-humanin-like peptide 2 (SHLP2) have been reported to act as insulin sensitizers and modulate metabolism.<h4>Objectives</h4>By using a metabolomic approach, this study explores how the plasma metabolite profile is regulated in response to humanin and SHLP2 treatment in a diet-induced obesity (DIO) mouse model. The results also shed light on the potential mechanism underlying MDPs' insulin sensitization effects.<h4>Methods</h4>Plasma samples were obtained from DIO mice subjected to vehicle (water) treatment, or peptide treatment with either humanin analog S14G (HNG) or SHLP2 (n = 6 per group). VeMetabolomics : Official journal of the Metabolomic SocietyMetabolomic profile of diet-induced obesity mice in response to humanin and small humanin-like peptide 2 treatment.PMC6554247PC160353P01AG034906RF1 AG061834T32 AG000037P01 AG055369R01AG034430Cohen PMehta HHXiao JYen KRamirez RKim SJMiller BfalseMetabolomic profile of diet-induced obesity mice in response to humanin and small humanin-like peptide 2 treatment.<h4>Introduction</h4>The mitochondrial-derived peptides (MDPs) are a novel group of natural occurring peptides that have important signaling functions and biological activity. Both humanin and small-humanin-like peptide 2 (SHLP2) have been reported to act as insulin sensitizers and modulate metabolism.<h4>Objectives</h4>By using a metabolomic approach, this study explores how the plasma metabolite profile is regulated in response to humanin and SHLP2 treatment in a diet-induced obesity (DIO) mouse model. The results also shed light on the potential mechanism underlying MDPs' insulin sensitization effects.<h4>Methods</h4>Plasma samples were obtained from DIO mice subjected to vehicle (water) treatment, or peptide treatment with either humanin analog S14G (HNG) or SHLP2 (n = 6 per group). Ve2019-01-01T00:00:00Z2019 Jun2025-04-04T11:06:56.368Z2019-07-24T07:11:16ZS-EPMC65542473117232810.1007/s11306-019-1549-7