{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang R"],"funding":["Center for Life Sciences","National Natural Science Foundation of China"],"pagination":["162-176"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6556835"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16"],"pubmed_abstract":["How actin-bundling factors cooperatively regulate shank-localized actin bundles remains largely unexplored. Here we demonstrate that FIM5 and PLIM2a/PLIM2b decorate shank-localized actin bundles and that loss of function of PLIM2a and/or PLIM2b suppresses phenotypes associated with fim5 mutants. Specifically, knockout of PLIM2a and/or PLIM2b partially suppresses the disorganized actin bundle and intracellular trafficking phenotype in fim5 pollen tubes. PLIM2a/PLIM2b generates thick but loosely packed actin bundles, whereas FIM5 generates thin but tight actin bundles that tend to be cross-linked into networks in vitro. Furthermore, PLIM2a/PLIM2b and FIM5 compete for binding to actin filaments in vitro, and PLIM2a/PLIM2b decorate disorganized actin bundles in fim5 pollen tubes. These data to"],"journal":["iScience"],"pubmed_title":["The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes."],"pmcid":["PMC6556835"],"funding_grant_id":["31471266","31671390"],"pubmed_authors":["Jiang Y","Huang S","Yu R","Dai A","Zhao W","Cao D","Qu X","Zhang M","Wang H","Zhang R","Lan Y"],"additional_accession":[]},"is_claimable":false,"name":"The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.","description":"How actin-bundling factors cooperatively regulate shank-localized actin bundles remains largely unexplored. Here we demonstrate that FIM5 and PLIM2a/PLIM2b decorate shank-localized actin bundles and that loss of function of PLIM2a and/or PLIM2b suppresses phenotypes associated with fim5 mutants. Specifically, knockout of PLIM2a and/or PLIM2b partially suppresses the disorganized actin bundle and intracellular trafficking phenotype in fim5 pollen tubes. PLIM2a/PLIM2b generates thick but loosely packed actin bundles, whereas FIM5 generates thin but tight actin bundles that tend to be cross-linked into networks in vitro. Furthermore, PLIM2a/PLIM2b and FIM5 compete for binding to actin filaments in vitro, and PLIM2a/PLIM2b decorate disorganized actin bundles in fim5 pollen tubes. These data to","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2025-04-04T22:08:28.321Z","creation":"2019-07-24T07:12:24Z"},"accession":"S-EPMC6556835","cross_references":{"pubmed":["31181400"],"doi":["10.1016/j.isci.2019.05.026"]}}