biostudies-literatureZhang RCenter for Life SciencesNational Natural Science Foundation of China162-176https://www.ebi.ac.uk/biostudies/studies/S-EPMC6556835biostudies-literatureUnknown16How actin-bundling factors cooperatively regulate shank-localized actin bundles remains largely unexplored. Here we demonstrate that FIM5 and PLIM2a/PLIM2b decorate shank-localized actin bundles and that loss of function of PLIM2a and/or PLIM2b suppresses phenotypes associated with fim5 mutants. Specifically, knockout of PLIM2a and/or PLIM2b partially suppresses the disorganized actin bundle and intracellular trafficking phenotype in fim5 pollen tubes. PLIM2a/PLIM2b generates thick but loosely packed actin bundles, whereas FIM5 generates thin but tight actin bundles that tend to be cross-linked into networks in vitro. Furthermore, PLIM2a/PLIM2b and FIM5 compete for binding to actin filaments in vitro, and PLIM2a/PLIM2b decorate disorganized actin bundles in fim5 pollen tubes. These data toiScienceThe Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.PMC65568353147126631671390Jiang YHuang SYu RDai AZhao WCao DQu XZhang MWang HZhang RLan YfalseThe Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes.How actin-bundling factors cooperatively regulate shank-localized actin bundles remains largely unexplored. Here we demonstrate that FIM5 and PLIM2a/PLIM2b decorate shank-localized actin bundles and that loss of function of PLIM2a and/or PLIM2b suppresses phenotypes associated with fim5 mutants. Specifically, knockout of PLIM2a and/or PLIM2b partially suppresses the disorganized actin bundle and intracellular trafficking phenotype in fim5 pollen tubes. PLIM2a/PLIM2b generates thick but loosely packed actin bundles, whereas FIM5 generates thin but tight actin bundles that tend to be cross-linked into networks in vitro. Furthermore, PLIM2a/PLIM2b and FIM5 compete for binding to actin filaments in vitro, and PLIM2a/PLIM2b decorate disorganized actin bundles in fim5 pollen tubes. These data to2019-01-01T00:00:00Z2019 Jun2025-04-04T22:08:28.321Z2019-07-24T07:12:24ZS-EPMC65568353118140010.1016/j.isci.2019.05.026