biostudies-literatureAhn JHNational Research Foundation of Korea8397https://www.ebi.ac.uk/biostudies/studies/S-EPMC6557891biostudies-literatureUnknown9(1)Inhibitor of kappa B (IκB)-ζ transcription is rapidly induced by stimulation with TLR ligands and IL-1. Despite high IκBζ expression in inflammation sites, the association of IκBζ with host defence via systemic immune responses against bacterial infection remains unclear. Oral immunisation with a recombinant attenuated Salmonella vaccine (RASV) strain did not protect IκBζ-deficient mice against a lethal Salmonella challenge. IκBζ-deficient mice failed to produce Salmonella LPS-specific IgG, especially IgG2a, although inflammatory cytokine production and immune cell infiltration into the liver increased after oral RASV administration. Moreover, IκBζ-deficient mice exhibited enhanced splenic germinal centre reactions followed by increased total IgG production, despite IκBζ-deficient B cells Scientific reportsIκBζ facilitates protective immunity against Salmonella infection via Th1 differentiation and IgG production.PMC6557891NRF-2017R1A2B2001963NRF-2016R1A4A1010115NRF-2017M3A9C8060387Ahn JHYang HYoon SIKwon BEChang SYKweon MNLee GSKo HJKang SGKim YICho JKim PHVallance BAfalseIκBζ facilitates protective immunity against Salmonella infection via Th1 differentiation and IgG production.Inhibitor of kappa B (IκB)-ζ transcription is rapidly induced by stimulation with TLR ligands and IL-1. Despite high IκBζ expression in inflammation sites, the association of IκBζ with host defence via systemic immune responses against bacterial infection remains unclear. Oral immunisation with a recombinant attenuated Salmonella vaccine (RASV) strain did not protect IκBζ-deficient mice against a lethal Salmonella challenge. IκBζ-deficient mice failed to produce Salmonella LPS-specific IgG, especially IgG2a, although inflammatory cytokine production and immune cell infiltration into the liver increased after oral RASV administration. Moreover, IκBζ-deficient mice exhibited enhanced splenic germinal centre reactions followed by increased total IgG production, despite IκBζ-deficient B cells 2019-01-01T00:00:00Z2019 Jun2025-04-04T22:09:25.949Z2019-07-24T07:13:56ZS-EPMC65578913118279010.1038/s41598-019-44019-3