{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Webb J"],"funding":["RCUK | Biotechnology and Biological Sciences Research Council","Biotechnology and Biological Sciences Research Council","Engineering and Physical Sciences Research Council"],"pagination":["e00187-19"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6561320"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["4(4)"],"pubmed_abstract":["Productivity of bacterial cell factories is frequently compromised by stresses imposed by recombinant protein synthesis and carbon-to-product conversion, but little is known about these bioprocesses at a systems level. Production of the unnatural metabolite citramalate in <i>Escherichia coli</i> requires the expression of a single gene coding for citramalate synthase. Multiomic analyses of a fermentation producing 25  g liter<sup>-1</sup> citramalate were undertaken to uncover the reasons for its productivity. Metabolite, transcript, protein, and lipid profiles of high-cell-density, fed-batch fermentations of <i>E. coli</i> expressing either citramalate synthase or an inactivated enzyme were similar. Both fermentations showed downregulation of flagellar genes and upregulation of chaperones"],"journal":["mSystems"],"pubmed_title":["Systems Analyses Reveal the Resilience of Escherichia coli Physiology during Accumulation and Export of the Nonnative Organic Acid Citramalate."],"pmcid":["PMC6561320"],"funding_grant_id":["BB/N01040X/1","BB/N010493/1","BB/N01037X/1","EP/K039660/1","EP/M028127/1"],"pubmed_authors":["Kelly DJ","Lilley K","Stephens G","Webb J","Langer S","Green J","Larson T","Thomas GH","Minde DP","Eastham G","Alstrom-Moore A","Rossoni L","Springthorpe V","Walker H"],"additional_accession":[]},"is_claimable":false,"name":"Systems Analyses Reveal the Resilience of Escherichia coli Physiology during Accumulation and Export of the Nonnative Organic Acid Citramalate.","description":"Productivity of bacterial cell factories is frequently compromised by stresses imposed by recombinant protein synthesis and carbon-to-product conversion, but little is known about these bioprocesses at a systems level. Production of the unnatural metabolite citramalate in <i>Escherichia coli</i> requires the expression of a single gene coding for citramalate synthase. Multiomic analyses of a fermentation producing 25  g liter<sup>-1</sup> citramalate were undertaken to uncover the reasons for its productivity. Metabolite, transcript, protein, and lipid profiles of high-cell-density, fed-batch fermentations of <i>E. coli</i> expressing either citramalate synthase or an inactivated enzyme were similar. Both fermentations showed downregulation of flagellar genes and upregulation of chaperones","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2026-06-14T05:32:49.642Z","creation":"2020-05-22T11:07:06Z"},"accession":"S-EPMC6561320","cross_references":{"pubmed":["31186337"],"doi":["10.1128/mSystems.00187-19"]}}