biostudies-literatureWebb JRCUK | Biotechnology and Biological Sciences Research CouncilBiotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research Councile00187-19https://www.ebi.ac.uk/biostudies/studies/S-EPMC6561320biostudies-literatureUnknown4(4)Productivity of bacterial cell factories is frequently compromised by stresses imposed by recombinant protein synthesis and carbon-to-product conversion, but little is known about these bioprocesses at a systems level. Production of the unnatural metabolite citramalate in <i>Escherichia coli</i> requires the expression of a single gene coding for citramalate synthase. Multiomic analyses of a fermentation producing 25  g liter^-1 citramalate were undertaken to uncover the reasons for its productivity. Metabolite, transcript, protein, and lipid profiles of high-cell-density, fed-batch fermentations of <i>E. coli</i> expressing either citramalate synthase or an inactivated enzyme were similar. Both fermentations showed downregulation of flagellar genes and upregulation of chaperonesmSystemsSystems Analyses Reveal the Resilience of Escherichia coli Physiology during Accumulation and Export of the Nonnative Organic Acid Citramalate.PMC6561320BB/N01040X/1BB/N010493/1BB/N01037X/1EP/K039660/1EP/M028127/1Kelly DJLilley KStephens GWebb JLanger SGreen JLarson TThomas GHMinde DPEastham GAlstrom-Moore ARossoni LSpringthorpe VWalker HfalseSystems Analyses Reveal the Resilience of Escherichia coli Physiology during Accumulation and Export of the Nonnative Organic Acid Citramalate.Productivity of bacterial cell factories is frequently compromised by stresses imposed by recombinant protein synthesis and carbon-to-product conversion, but little is known about these bioprocesses at a systems level. Production of the unnatural metabolite citramalate in <i>Escherichia coli</i> requires the expression of a single gene coding for citramalate synthase. Multiomic analyses of a fermentation producing 25  g liter^-1 citramalate were undertaken to uncover the reasons for its productivity. Metabolite, transcript, protein, and lipid profiles of high-cell-density, fed-batch fermentations of <i>E. coli</i> expressing either citramalate synthase or an inactivated enzyme were similar. Both fermentations showed downregulation of flagellar genes and upregulation of chaperones2019-01-01T00:00:00Z2019 Jun2026-06-14T05:32:49.642Z2020-05-22T11:07:06ZS-EPMC65613203118633710.1128/mSystems.00187-19