{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang EJ"],"funding":["U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences","NSF | BIO | Division of Integrative Organismal Systems (IOS)","NSF | BIO | Division of Integrative Organismal Systems","NIGMS NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)","NIH HHS"],"pagination":["2630"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6570768"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-l"],"journal":["Nature communications"],"pubmed_title":["NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches."],"pmcid":["PMC6570768"],"funding_grant_id":["R01GM087388","R01 GM087388","IOS-1051602","S10 OD016290","R01 GM100051"],"pubmed_authors":["Liu J","Yoo CY","Weigel D","Sun TP","Zhou P","Cao J","Chen M","Yang EJ","Wang H","Li FW","Pryer KM"],"additional_accession":[]},"is_claimable":false,"name":"NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.","description":"Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-l","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2026-04-29T03:49:11.779Z","creation":"2025-05-31T22:57:02.949Z"},"accession":"S-EPMC6570768","cross_references":{"pubmed":["31201314"],"doi":["10.1038/s41467-019-10517-1"]}}