{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Y"],"funding":["Guangzhou Science and Technology Project","Guangdong Provincial Applied Science and Technology Research &amp; Development Program","National Natural Science Foundation of China"],"pagination":["12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6570921"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["We previously identified a <i>TCR Vβ</i>21 T cell clone which was specific to CML patients, and demonstrated that <i>TCR Vα</i>13/<i>β</i>21 gene-modified CD3<sup>+</sup> T cells had specific cytotoxicity for HLA-A11<sup>+</sup> K562 cells. However, it remains unclear which antigen is specifically recognized by the <i>TCR Vβ</i>21 T cell clone. In this study, CD3<sup>+</sup> T cells from healthy donor peripheral blood were stimulated with the WT1 peptide or mixed BCR-ABL peptides in the presence or absence of IL-2 and IL-7. The distribution of the <i>TCR Vβ</i> repertoire was analyzed after different stimulations. We found that the mixed BCR-ABL peptides induced clonally expanded <i>Vβ</i>7-9-<i>Dβ</i>2-<i>Jβ</i>2-7 T cells while the Wilms Tumor 1 peptide induced clonally expanded <i>Vβ</i"],"journal":["Biomarker research"],"pubmed_title":["Identification of <i>TCR Vβ</i>11-2-<i>Dβ</i>1-<i>Jβ</i>1-1 T cell clone specific for WT1 peptides using high-throughput <i>TCRβ</i> gene sequencing."],"pmcid":["PMC6570921"],"funding_grant_id":["20180710004","201510010211","2016B020237006","91642111","81770152","201803040017"],"pubmed_authors":["Xu L","Li Y","Zhang Y","Wei W","Chen S","Zha X"],"additional_accession":[]},"is_claimable":false,"name":"Identification of <i>TCR Vβ</i>11-2-<i>Dβ</i>1-<i>Jβ</i>1-1 T cell clone specific for WT1 peptides using high-throughput <i>TCRβ</i> gene sequencing.","description":"We previously identified a <i>TCR Vβ</i>21 T cell clone which was specific to CML patients, and demonstrated that <i>TCR Vα</i>13/<i>β</i>21 gene-modified CD3<sup>+</sup> T cells had specific cytotoxicity for HLA-A11<sup>+</sup> K562 cells. However, it remains unclear which antigen is specifically recognized by the <i>TCR Vβ</i>21 T cell clone. In this study, CD3<sup>+</sup> T cells from healthy donor peripheral blood were stimulated with the WT1 peptide or mixed BCR-ABL peptides in the presence or absence of IL-2 and IL-7. The distribution of the <i>TCR Vβ</i> repertoire was analyzed after different stimulations. We found that the mixed BCR-ABL peptides induced clonally expanded <i>Vβ</i>7-9-<i>Dβ</i>2-<i>Jβ</i>2-7 T cells while the Wilms Tumor 1 peptide induced clonally expanded <i>Vβ</i","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-26T02:07:37.41Z","creation":"2019-07-24T07:18:57Z"},"accession":"S-EPMC6570921","cross_references":{"pubmed":["31223481"],"doi":["10.1186/s40364-019-0163-1"]}}