biostudies-literatureZhang YGuangzhou Science and Technology ProjectGuangdong Provincial Applied Science and Technology Research & Development ProgramNational Natural Science Foundation of China12https://www.ebi.ac.uk/biostudies/studies/S-EPMC6570921biostudies-literatureUnknown7We previously identified a TCR Vβ21 T cell clone which was specific to CML patients, and demonstrated that TCR Vα13/β21 gene-modified CD3+ T cells had specific cytotoxicity for HLA-A11+ K562 cells. However, it remains unclear which antigen is specifically recognized by the TCR Vβ21 T cell clone. In this study, CD3+ T cells from healthy donor peripheral blood were stimulated with the WT1 peptide or mixed BCR-ABL peptides in the presence or absence of IL-2 and IL-7. The distribution of the TCR Vβ repertoire was analyzed after different stimulations. We found that the mixed BCR-ABL peptides induced clonally expanded Vβ7-9-Dβ2-Jβ2-7 T cells while the Wilms Tumor 1 peptide induced clonally expanded Vβ</iBiomarker researchIdentification of TCR Vβ11-2-Dβ1-Jβ1-1 T cell clone specific for WT1 peptides using high-throughput TCRβ gene sequencing.PMC6570921201807100042015100102112016B0202370069164211181770152201803040017Xu LLi YZhang YWei WChen SZha XfalseIdentification of TCR Vβ11-2-Dβ1-Jβ1-1 T cell clone specific for WT1 peptides using high-throughput TCRβ gene sequencing.We previously identified a TCR Vβ21 T cell clone which was specific to CML patients, and demonstrated that TCR Vα13/β21 gene-modified CD3+ T cells had specific cytotoxicity for HLA-A11+ K562 cells. However, it remains unclear which antigen is specifically recognized by the TCR Vβ21 T cell clone. In this study, CD3+ T cells from healthy donor peripheral blood were stimulated with the WT1 peptide or mixed BCR-ABL peptides in the presence or absence of IL-2 and IL-7. The distribution of the TCR Vβ repertoire was analyzed after different stimulations. We found that the mixed BCR-ABL peptides induced clonally expanded Vβ7-9-Dβ2-Jβ2-7 T cells while the Wilms Tumor 1 peptide induced clonally expanded Vβ</i2019-01-01T00:00:00Z20192025-04-26T02:07:37.41Z2019-07-24T07:18:57ZS-EPMC65709213122348110.1186/s40364-019-0163-1