{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao W"],"funding":["National Natural Science Foundation of China"],"pagination":["e7111"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6571133"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["<h4>Background and objectives</h4>Hyperpolarization-activation cyclic nucleotide-gated (HCN) channels contribute to the effects of lidocaine. Capsazepine (CPZ), a competitive inhibitor of capsaicin of transient receptor potential vanilloid-1 channel, has also been found to inhibit HCN channel currents (<i>I</i> <sub>h</sub>). This study was designed to investigate whether CPZ could prolong durations of lidocaine in regional anesthesia.<h4>Methods</h4>Mouse HCN1 and HCN2 channels were expressed in human embryonic kidney 293 (HEK 293) cells. The effect of CPZ on <i>I</i> <sub>h</sub> was measured by whole-cell patch-clamping recording. Sciatic nerve block model in mice was used for the study in vivo. The mice were randomly divided into seven groups, respectively, receiving lidocaine, CPZ, ZD"],"journal":["PeerJ"],"pubmed_title":["Capsazepine prolongation of the duration of lidocaine block of sensory transmission in mice may be mediated by modulation of HCN channel currents."],"pmcid":["PMC6571133"],"funding_grant_id":["81771456 (to Cheng Zhou) and 81571353 (to Jin Liu)"],"pubmed_authors":["Li H","Liu J","Zhou C","Zhao W","Liao D","Li Q","Chen X","Liang P"],"additional_accession":[]},"is_claimable":false,"name":"Capsazepine prolongation of the duration of lidocaine block of sensory transmission in mice may be mediated by modulation of HCN channel currents.","description":"<h4>Background and objectives</h4>Hyperpolarization-activation cyclic nucleotide-gated (HCN) channels contribute to the effects of lidocaine. Capsazepine (CPZ), a competitive inhibitor of capsaicin of transient receptor potential vanilloid-1 channel, has also been found to inhibit HCN channel currents (<i>I</i> <sub>h</sub>). This study was designed to investigate whether CPZ could prolong durations of lidocaine in regional anesthesia.<h4>Methods</h4>Mouse HCN1 and HCN2 channels were expressed in human embryonic kidney 293 (HEK 293) cells. The effect of CPZ on <i>I</i> <sub>h</sub> was measured by whole-cell patch-clamping recording. Sciatic nerve block model in mice was used for the study in vivo. The mice were randomly divided into seven groups, respectively, receiving lidocaine, CPZ, ZD","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-04T22:09:17.012Z","creation":"2019-07-24T07:19:03Z"},"accession":"S-EPMC6571133","cross_references":{"pubmed":["31223538"],"doi":["10.7717/peerj.7111"]}}