biostudies-literatureZhao WNational Natural Science Foundation of Chinae7111https://www.ebi.ac.uk/biostudies/studies/S-EPMC6571133biostudies-literatureUnknown7<h4>Background and objectives</h4>Hyperpolarization-activation cyclic nucleotide-gated (HCN) channels contribute to the effects of lidocaine. Capsazepine (CPZ), a competitive inhibitor of capsaicin of transient receptor potential vanilloid-1 channel, has also been found to inhibit HCN channel currents (<i>I</i> _h). This study was designed to investigate whether CPZ could prolong durations of lidocaine in regional anesthesia.<h4>Methods</h4>Mouse HCN1 and HCN2 channels were expressed in human embryonic kidney 293 (HEK 293) cells. The effect of CPZ on <i>I</i> _h was measured by whole-cell patch-clamping recording. Sciatic nerve block model in mice was used for the study in vivo. The mice were randomly divided into seven groups, respectively, receiving lidocaine, CPZ, ZDPeerJCapsazepine prolongation of the duration of lidocaine block of sensory transmission in mice may be mediated by modulation of HCN channel currents.PMC657113381771456 (to Cheng Zhou) and 81571353 (to Jin Liu)Li HLiu JZhou CZhao WLiao DLi QChen XLiang PfalseCapsazepine prolongation of the duration of lidocaine block of sensory transmission in mice may be mediated by modulation of HCN channel currents.<h4>Background and objectives</h4>Hyperpolarization-activation cyclic nucleotide-gated (HCN) channels contribute to the effects of lidocaine. Capsazepine (CPZ), a competitive inhibitor of capsaicin of transient receptor potential vanilloid-1 channel, has also been found to inhibit HCN channel currents (<i>I</i> _h). This study was designed to investigate whether CPZ could prolong durations of lidocaine in regional anesthesia.<h4>Methods</h4>Mouse HCN1 and HCN2 channels were expressed in human embryonic kidney 293 (HEK 293) cells. The effect of CPZ on <i>I</i> _h was measured by whole-cell patch-clamping recording. Sciatic nerve block model in mice was used for the study in vivo. The mice were randomly divided into seven groups, respectively, receiving lidocaine, CPZ, ZD2019-01-01T00:00:00Z20192025-04-04T22:09:17.012Z2019-07-24T07:19:03ZS-EPMC65711333122353810.7717/peerj.7111