{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["116(24)"],"submitter":["Pizzolato G"],"pubmed_abstract":["γδ T lymphocytes represent ∼1% of human peripheral blood mononuclear cells and even more cells in most tissues of vertebrates. Although they have important anticancer functions, most current single-cell RNA sequencing (scRNA-seq) studies do not identify γδ T lymphocytes because their transcriptomes at the single-cell level are unknown. Here we show that high-resolution clustering of large scRNA-seq datasets and a combination of gene signatures allow the specific detection of human γδ T lymphocytes and identification of their T cell receptor (TCR)Vδ1 and TCRVδ2 subsets in large datasets from complex cell mixtures. In <i>t</i>-distributed stochastic neighbor embedding plots from blood and tumor samples, the few γδ T lymphocytes appear collectively embedded between cytotoxic CD8 T and NK cell"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["11906-11915"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6576116"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell RNA sequencing unveils the shared and the distinct cytotoxic hallmarks of human TCRVδ1 and TCRVδ2 γδ T lymphocytes."],"pmcid":["PMC6576116"],"pubmed_authors":["Meraviglia S","Fournie JJ","Kaminski H","Labourdette D","Quillet-Mary A","Pizzolato G","Cadot S","Ysebaert L","Martins F","Merville P","Dechanet-Merville J","Franchini DM","Dieli F","Pont F","Tosolini M","Poupot M","Laurent C","Valle C","Milpied P"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing unveils the shared and the distinct cytotoxic hallmarks of human TCRVδ1 and TCRVδ2 γδ T lymphocytes.","description":"γδ T lymphocytes represent ∼1% of human peripheral blood mononuclear cells and even more cells in most tissues of vertebrates. Although they have important anticancer functions, most current single-cell RNA sequencing (scRNA-seq) studies do not identify γδ T lymphocytes because their transcriptomes at the single-cell level are unknown. Here we show that high-resolution clustering of large scRNA-seq datasets and a combination of gene signatures allow the specific detection of human γδ T lymphocytes and identification of their T cell receptor (TCR)Vδ1 and TCRVδ2 subsets in large datasets from complex cell mixtures. In <i>t</i>-distributed stochastic neighbor embedding plots from blood and tumor samples, the few γδ T lymphocytes appear collectively embedded between cytotoxic CD8 T and NK cell","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2026-04-29T13:02:10.965Z","creation":"2020-05-21T19:56:30Z"},"accession":"S-EPMC6576116","cross_references":{"pubmed":["31118283"],"doi":["10.1073/pnas.1818488116"]}}