{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han M"],"funding":["National Institute of General Medical Sciences","NIH HHS","NIGMS NIH HHS"],"pagination":["500"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6580472"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(1)"],"pubmed_abstract":["<h4>Background</h4>The wide variety of specialized permissive and repressive mechanisms by which germ cells regulate developmental gene expression are not well understood genome-wide. Isolation of germ cells with high integrity and purity from living animals is necessary to address these open questions, but no straightforward methods are currently available.<h4>Results</h4>Here we present an experimental paradigm that permits the isolation of nuclei from C. elegans germ cells at quantities sufficient for genomic analyses. We demonstrate that these nuclei represent a very pure population and are suitable for both transcriptome analysis (RNA-seq) and chromatin immunoprecipitation (ChIP-seq) of histone modifications. From these data, we find unexpected germline- and soma-specific patterns of "],"journal":["BMC genomics"],"pubmed_title":["Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression."],"pmcid":["PMC6580472"],"funding_grant_id":["R35 GM131776","R01 GM108663","P40 OD010440","R01GM108663"],"pubmed_authors":["Han M","Reinke V","Wei G","McManus CE","Hillier LW"],"additional_accession":[]},"is_claimable":false,"name":"Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression.","description":"<h4>Background</h4>The wide variety of specialized permissive and repressive mechanisms by which germ cells regulate developmental gene expression are not well understood genome-wide. Isolation of germ cells with high integrity and purity from living animals is necessary to address these open questions, but no straightforward methods are currently available.<h4>Results</h4>Here we present an experimental paradigm that permits the isolation of nuclei from C. elegans germ cells at quantities sufficient for genomic analyses. We demonstrate that these nuclei represent a very pure population and are suitable for both transcriptome analysis (RNA-seq) and chromatin immunoprecipitation (ChIP-seq) of histone modifications. From these data, we find unexpected germline- and soma-specific patterns of ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2026-07-14T15:02:24.364Z","creation":"2019-07-24T07:20:32Z"},"accession":"S-EPMC6580472","cross_references":{"pubmed":["31208332"],"doi":["10.1186/s12864-019-5893-9"]}}