{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tan S"],"funding":["National Natural Science Foundation of China","Natural Science Foundation of Hunan Province"],"pagination":["42"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6580496"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24"],"pubmed_abstract":["Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H<sub>2</sub>O<sub>2</sub>. Apoptosis of HBE-Dp71AS cells induced by H<sub>2</sub>O<sub>2</sub> was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinas"],"journal":["Cellular & molecular biology letters"],"pubmed_title":["Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H<sub>2</sub>O<sub>2</sub>."],"pmcid":["PMC6580496"],"funding_grant_id":["81870071","06C0410"],"pubmed_authors":["Zhao S","Xiao L","Xie J","Xiao X","Tan S"],"additional_accession":[]},"is_claimable":false,"name":"Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H<sub>2</sub>O<sub>2</sub>.","description":"Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H<sub>2</sub>O<sub>2</sub>. Apoptosis of HBE-Dp71AS cells induced by H<sub>2</sub>O<sub>2</sub> was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinas","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-04-08T05:24:40.042Z","creation":"2019-07-24T07:20:31Z"},"accession":"S-EPMC6580496","cross_references":{"pubmed":["31236120"],"doi":["10.1186/s11658-019-0169-6"]}}