biostudies-literatureTan SNational Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province42https://www.ebi.ac.uk/biostudies/studies/S-EPMC6580496biostudies-literatureUnknown24Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H<sub>2</sub>O<sub>2</sub>. Apoptosis of HBE-Dp71AS cells induced by H<sub>2</sub>O<sub>2</sub> was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinasCellular & molecular biology lettersDp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H<sub>2</sub>O<sub>2</sub>.PMC65804968187007106C0410Zhao SXiao LXie JXiao XTan SfalseDp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H<sub>2</sub>O<sub>2</sub>.Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H<sub>2</sub>O<sub>2</sub>. Apoptosis of HBE-Dp71AS cells induced by H<sub>2</sub>O<sub>2</sub> was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinas2019-01-01T00:00:00Z20192026-04-08T05:24:40.042Z2019-07-24T07:20:31ZS-EPMC65804963123612010.1186/s11658-019-0169-6