biostudies-literatureAndries VStichting Tegen KankerFederaal WetenschapsbeleidFonds Wetenschappelijk OnderzoekUniversiteit GentFederaal Wetenschapsbeleid (BE)598https://www.ebi.ac.uk/biostudies/studies/S-EPMC6580527biostudies-literatureUnknown19(1)<h4>Background</h4>NANOS3 is a gene conserved throughout evolution. Despite the quite low conservation of Nanos sequences between different organisms and even between Nanos paralogs, their role in germ cell development is remarkably universal. Human Nanos3 expression is normally restricted to the gonads and the brain. However, ectopic activation of this gene has been detected in various human cancers. Until now, Nanos3 and other Nanos proteins have been studied almost exclusively in germ cell development.<h4>Methods</h4>Transgenic mice were generated by targeted insertion of a human Nanos3 cDNA into the ROSA26 locus. The transgene could be spatiotemporally induced by Cre recombinase activity removing an upstream floxed STOP cassette. A lung tumor model with ectopic Nanos3 expression was baBMC cancerA new mouse model to study the role of ectopic Nanos3 expression in cancer.PMC65805272012-191B/13590IAP7/07G.0235.10NConcerted Research Actions BOF08/GOA/019Andries VHochepied TLemeire KStaes KBirembaut PBerx GTaminau Jvan Roy FDe Keuckelaere EfalseA new mouse model to study the role of ectopic Nanos3 expression in cancer.<h4>Background</h4>NANOS3 is a gene conserved throughout evolution. Despite the quite low conservation of Nanos sequences between different organisms and even between Nanos paralogs, their role in germ cell development is remarkably universal. Human Nanos3 expression is normally restricted to the gonads and the brain. However, ectopic activation of this gene has been detected in various human cancers. Until now, Nanos3 and other Nanos proteins have been studied almost exclusively in germ cell development.<h4>Methods</h4>Transgenic mice were generated by targeted insertion of a human Nanos3 cDNA into the ROSA26 locus. The transgene could be spatiotemporally induced by Cre recombinase activity removing an upstream floxed STOP cassette. A lung tumor model with ectopic Nanos3 expression was ba2019-01-01T00:00:00Z2019 Jun2025-04-18T13:30:40.975Z2019-07-24T07:20:37ZS-EPMC65805273120837310.1186/s12885-019-5807-x