biostudies-literatureLiu YNational Key Basic Research Program of ChinaKey Project of National Natural Science Foundation of ChinaNational Nonprofit Institute Research GrantProgram of New Breed Development via Transgenic TechnologyNational Natural Science Foundation of ChinaFoshan University Initiative Scientific Research Program63-74https://www.ebi.ac.uk/biostudies/studies/S-EPMC6587779biostudies-literatureUnknown86(1)Cumulus cells secreting steroid hormones have important functions in oocyte development. Several members of the short-chain dehydrogenase/reductase (SDR) family are critical to the biosynthesis of steroid hormones. NADPH-dependent retinol dehydrogenase/reductase ( NRDR), a member of the SDR superfamily, is overexpressed in pig breeds that also show high levels of androstenone. However, the potential functions and regulatory mechanisms of NRDR in pig ovaries have not been reported to date. The present study demonstrated that NRDR is highly expressed in pig ovaries and is specifically located in cumulus granulosa cells. Functional studies showed that NRDR inhibition increased estradiol synthesis. Both pregnant mare serum gonadotropin and human chorionic gonadotropin downregulated the expressMolecular reproduction and developmentNRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs.PMC65877792018-YWF-YB-731330074Y2016JC072015CB9431012016ZX080011-006Li WLiu YLi HYang YZhang YZhou RAo HGeng ZLi KfalseNRDR inhibits estradiol synthesis and is associated with changes in reproductive traits in pigs.Cumulus cells secreting steroid hormones have important functions in oocyte development. Several members of the short-chain dehydrogenase/reductase (SDR) family are critical to the biosynthesis of steroid hormones. NADPH-dependent retinol dehydrogenase/reductase ( NRDR), a member of the SDR superfamily, is overexpressed in pig breeds that also show high levels of androstenone. However, the potential functions and regulatory mechanisms of NRDR in pig ovaries have not been reported to date. The present study demonstrated that NRDR is highly expressed in pig ovaries and is specifically located in cumulus granulosa cells. Functional studies showed that NRDR inhibition increased estradiol synthesis. Both pregnant mare serum gonadotropin and human chorionic gonadotropin downregulated the express2019-01-01T00:00:00Z2019 Jan2025-06-01T00:30:12.698Z2025-06-01T00:30:12.698ZS-EPMC65877793037255110.1002/mrd.23080