biostudies-literatureJia Dthe National Key S&amp;T Special Projects on Major Infectious DiseasesBeijing Municipal Science &amp; Technology Projectthe National Natural Science Foundation of China83https://www.ebi.ac.uk/biostudies/studies/S-EPMC6589171biostudies-literatureUnknown16(1)<h4>Background</h4>Shenzhen City is a rapidly growing area with a large number of floating populations, thus making it difficult to control HIV. Serial cross-sectional studies are helpful for the prediction of epidemiological tendency. In this study, two parallel cross-sectional studies were compared to explore changes in HIV epidemiology in Shenzhen, China.<h4>Methods</h4>Two hundred and fifty newly reported HIV-positive cases were randomly selected in Shenzhen City in 2013 and 2015. Socio-demographical information was collected with informed consent. Full-length gag and partial pol genes were amplified using nested RT-PCR followed by sequencing and phylogenetic analysis. The genotypes of anti-HIV drug resistance were also analyzed. The characteristics of the HIV epidemics of 2013 and 201Virology journalTwo-year cross-sectional studies reveal that single, young MSMs in Shenzhen, China are at high risk for HIV infection.PMC658917181273137D14110000031400181773493815732112012ZX10002Li HLi JJia DChen LJia LLi LGui TLiu YLi TZheng CWang XHan JZhao JfalseTwo-year cross-sectional studies reveal that single, young MSMs in Shenzhen, China are at high risk for HIV infection.<h4>Background</h4>Shenzhen City is a rapidly growing area with a large number of floating populations, thus making it difficult to control HIV. Serial cross-sectional studies are helpful for the prediction of epidemiological tendency. In this study, two parallel cross-sectional studies were compared to explore changes in HIV epidemiology in Shenzhen, China.<h4>Methods</h4>Two hundred and fifty newly reported HIV-positive cases were randomly selected in Shenzhen City in 2013 and 2015. Socio-demographical information was collected with informed consent. Full-length gag and partial pol genes were amplified using nested RT-PCR followed by sequencing and phylogenetic analysis. The genotypes of anti-HIV drug resistance were also analyzed. The characteristics of the HIV epidemics of 2013 and 2012019-01-01T00:00:00Z2019 Jun2026-05-07T05:20:14.309Z2019-07-24T07:26:35ZS-EPMC65891713122895810.1186/s12985-019-1189-6