{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou XL"],"funding":["China Agriculture Research System","National Natural Science Foundation of China"],"pagination":["1498-1512"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6592228"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(13)"],"pubmed_abstract":["Geminin is a master regulator of cell-cycle progression that ensures the timely onset of DNA replication and prevents re-replication in vertebrates and invertebrates. Previously, we identified two <i>Geminin</i> genes, <i>BmGeminin1</i> and <i>BmGeminn2</i>, in the silkworm <i>Bombyx mori</i>, and we found that RNA interference of <i>BmGeminin1</i> led to re-replication. However, the function of <i>BmGeminin2</i> remains poorly understood. In this study, we found that knockdown of <i>BmGeminin2</i> can improve cell proliferation, and upregulated G2/M-associated gene-<i>cyclinB</i>/<i>CDK1</i> expression. Then, we performed yeast two-hybrid screening to identify interacting proteins. Our results yielded 23 interacting proteins, which are involved in DNA replication, chromosome stabilization"],"journal":["Cell cycle (Georgetown, Tex.)"],"pubmed_title":["<i>BmGeminin2</i> interacts with <i>BmRRS1</i> and regulates <i>Bombyx mori</i> cell proliferation."],"pmcid":["PMC6592228"],"funding_grant_id":["31602009","CARS-18","31472152","31572466"],"pubmed_authors":["Chen P","Pan MH","Tang XF","Chen XY","Lu C","Zhou XL","Dong ZQ","Wei Y","Zhang Q"],"additional_accession":[]},"is_claimable":false,"name":"<i>BmGeminin2</i> interacts with <i>BmRRS1</i> and regulates <i>Bombyx mori</i> cell proliferation.","description":"Geminin is a master regulator of cell-cycle progression that ensures the timely onset of DNA replication and prevents re-replication in vertebrates and invertebrates. Previously, we identified two <i>Geminin</i> genes, <i>BmGeminin1</i> and <i>BmGeminn2</i>, in the silkworm <i>Bombyx mori</i>, and we found that RNA interference of <i>BmGeminin1</i> led to re-replication. However, the function of <i>BmGeminin2</i> remains poorly understood. In this study, we found that knockdown of <i>BmGeminin2</i> can improve cell proliferation, and upregulated G2/M-associated gene-<i>cyclinB</i>/<i>CDK1</i> expression. Then, we performed yeast two-hybrid screening to identify interacting proteins. Our results yielded 23 interacting proteins, which are involved in DNA replication, chromosome stabilization","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2026-05-01T13:47:32.848Z","creation":"2020-06-02T07:03:28Z"},"accession":"S-EPMC6592228","cross_references":{"pubmed":["31145019"],"doi":["10.1080/15384101.2019.1624109"]}}