biostudies-literatureZhou XLChina Agriculture Research SystemNational Natural Science Foundation of China1498-1512https://www.ebi.ac.uk/biostudies/studies/S-EPMC6592228biostudies-literatureUnknown18(13)Geminin is a master regulator of cell-cycle progression that ensures the timely onset of DNA replication and prevents re-replication in vertebrates and invertebrates. Previously, we identified two <i>Geminin</i> genes, <i>BmGeminin1</i> and <i>BmGeminn2</i>, in the silkworm <i>Bombyx mori</i>, and we found that RNA interference of <i>BmGeminin1</i> led to re-replication. However, the function of <i>BmGeminin2</i> remains poorly understood. In this study, we found that knockdown of <i>BmGeminin2</i> can improve cell proliferation, and upregulated G2/M-associated gene-<i>cyclinB</i>/<i>CDK1</i> expression. Then, we performed yeast two-hybrid screening to identify interacting proteins. Our results yielded 23 interacting proteins, which are involved in DNA replication, chromosome stabilizationCell cycle (Georgetown, Tex.)<i>BmGeminin2</i> interacts with <i>BmRRS1</i> and regulates <i>Bombyx mori</i> cell proliferation.PMC659222831602009CARS-183147215231572466Chen PPan MHTang XFChen XYLu CZhou XLDong ZQWei YZhang Qfalse<i>BmGeminin2</i> interacts with <i>BmRRS1</i> and regulates <i>Bombyx mori</i> cell proliferation.Geminin is a master regulator of cell-cycle progression that ensures the timely onset of DNA replication and prevents re-replication in vertebrates and invertebrates. Previously, we identified two <i>Geminin</i> genes, <i>BmGeminin1</i> and <i>BmGeminn2</i>, in the silkworm <i>Bombyx mori</i>, and we found that RNA interference of <i>BmGeminin1</i> led to re-replication. However, the function of <i>BmGeminin2</i> remains poorly understood. In this study, we found that knockdown of <i>BmGeminin2</i> can improve cell proliferation, and upregulated G2/M-associated gene-<i>cyclinB</i>/<i>CDK1</i> expression. Then, we performed yeast two-hybrid screening to identify interacting proteins. Our results yielded 23 interacting proteins, which are involved in DNA replication, chromosome stabilization2019-01-01T00:00:00Z2019 Jul2026-05-01T13:47:32.848Z2020-06-02T07:03:28ZS-EPMC65922283114501910.1080/15384101.2019.1624109