{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jaderson M"],"funding":["National Institute of Environmental Health Sciences","National Institute for Occupational Safety and Health"],"pagination":["196-204"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6598797"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(2)"],"pubmed_abstract":["Background
Liquid chromatography-tandem mass spectrometry (LC-MSMS) for simultaneous analysis of multiple microbial secondary metabolites (MSMs) is potentially subject to interference by matrix components.Methods
We examined potential matrix effects (MEs) in analyses of 31 MSMs using ultraperformance LC-MSMS. Twenty-one dust aliquots from three buildings (seven aliquots/building) were spiked with seven concentrations of each of the MSMs (6.2 pg/μl-900 pg/μl) and then extracted. Another set of 21 aliquots were first extracted and then, the extract was spiked with the same concentrations. We added deepoxy-deoxynivalenol (DOM) to all aliquots as a universal internal standard. Ten microliters of the extract was injected into the ultraperformance LC-MSMS. ME was calculated by su"],"journal":["Safety and health at work"],"pubmed_title":["Evaluation of Matrix Effects in Quantifying Microbial Secondary Metabolites in Indoor Dust Using Ultraperformance Liquid Chromatograph-Tandem Mass Spectrometer."],"pmcid":["PMC6598797"],"funding_grant_id":["AES12007001-1-0-6"],"pubmed_authors":["Jaderson M","Park JH"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of Matrix Effects in Quantifying Microbial Secondary Metabolites in Indoor Dust Using Ultraperformance Liquid Chromatograph-Tandem Mass Spectrometer.","description":"Background
Liquid chromatography-tandem mass spectrometry (LC-MSMS) for simultaneous analysis of multiple microbial secondary metabolites (MSMs) is potentially subject to interference by matrix components.Methods
We examined potential matrix effects (MEs) in analyses of 31 MSMs using ultraperformance LC-MSMS. Twenty-one dust aliquots from three buildings (seven aliquots/building) were spiked with seven concentrations of each of the MSMs (6.2 pg/μl-900 pg/μl) and then extracted. Another set of 21 aliquots were first extracted and then, the extract was spiked with the same concentrations. We added deepoxy-deoxynivalenol (DOM) to all aliquots as a universal internal standard. Ten microliters of the extract was injected into the ultraperformance LC-MSMS. ME was calculated by su","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2025-04-22T02:32:04.809Z","creation":"2019-07-24T07:32:38Z"},"accession":"S-EPMC6598797","cross_references":{"pubmed":["31297282"],"doi":["10.1016/j.shaw.2018.12.004"]}}