{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu L"],"funding":["National Water Pollution Control and Treatment Science and Technology Major Project"],"pagination":["e7188"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6599450"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["In this study, a SYBR Green quantitative real-time PCR method was established and applied. Relative expression of the synthetic genes from <i>Microcystis</i> gas vesicles (<i>gvpC</i>), algal toxin genes (<i>mcyA</i>), and polysaccharides (<i>espL</i>) from water and sediments of Meiliang Bay and from the center of Lake Taihu were tested from January to June, 2017. Indoor <i>Microcystis aeruginosa</i> was used as the control group. The kit for total RNA extraction in <i>Microcystis</i> was optimized. Results showed that the optimized kit extracted high-concentrations and high-quality total RNA from <i>Microcystis</i>. The extraction purity and concentration were significantly higher than those extracted by the original kit. The transcription level of <i>gvpC</i> increased gradually until a"],"journal":["PeerJ"],"pubmed_title":["Recruitment of cyanobacteria by reverse transcription quantitative real-time PCR based on expression of <i>Microcystis</i> gene."],"pmcid":["PMC6599450"],"funding_grant_id":["2018ZX07208-004, 2017ZX07204-5"],"pubmed_authors":["Yu L","Shi L","Zhang M","Wu X","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Recruitment of cyanobacteria by reverse transcription quantitative real-time PCR based on expression of <i>Microcystis</i> gene.","description":"In this study, a SYBR Green quantitative real-time PCR method was established and applied. Relative expression of the synthetic genes from <i>Microcystis</i> gas vesicles (<i>gvpC</i>), algal toxin genes (<i>mcyA</i>), and polysaccharides (<i>espL</i>) from water and sediments of Meiliang Bay and from the center of Lake Taihu were tested from January to June, 2017. Indoor <i>Microcystis aeruginosa</i> was used as the control group. The kit for total RNA extraction in <i>Microcystis</i> was optimized. Results showed that the optimized kit extracted high-concentrations and high-quality total RNA from <i>Microcystis</i>. The extraction purity and concentration were significantly higher than those extracted by the original kit. The transcription level of <i>gvpC</i> increased gradually until a","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-04-29T03:22:58.606Z","creation":"2019-07-25T07:00:25Z"},"accession":"S-EPMC6599450","cross_references":{"pubmed":["31289701"],"doi":["10.7717/peerj.7188"]}}