biostudies-literatureYu LNational Water Pollution Control and Treatment Science and Technology Major Projecte7188https://www.ebi.ac.uk/biostudies/studies/S-EPMC6599450biostudies-literatureUnknown7In this study, a SYBR Green quantitative real-time PCR method was established and applied. Relative expression of the synthetic genes from <i>Microcystis</i> gas vesicles (<i>gvpC</i>), algal toxin genes (<i>mcyA</i>), and polysaccharides (<i>espL</i>) from water and sediments of Meiliang Bay and from the center of Lake Taihu were tested from January to June, 2017. Indoor <i>Microcystis aeruginosa</i> was used as the control group. The kit for total RNA extraction in <i>Microcystis</i> was optimized. Results showed that the optimized kit extracted high-concentrations and high-quality total RNA from <i>Microcystis</i>. The extraction purity and concentration were significantly higher than those extracted by the original kit. The transcription level of <i>gvpC</i> increased gradually until aPeerJRecruitment of cyanobacteria by reverse transcription quantitative real-time PCR based on expression of <i>Microcystis</i> gene.PMC65994502018ZX07208-004, 2017ZX07204-5Yu LShi LZhang MWu XYu YfalseRecruitment of cyanobacteria by reverse transcription quantitative real-time PCR based on expression of <i>Microcystis</i> gene.In this study, a SYBR Green quantitative real-time PCR method was established and applied. Relative expression of the synthetic genes from <i>Microcystis</i> gas vesicles (<i>gvpC</i>), algal toxin genes (<i>mcyA</i>), and polysaccharides (<i>espL</i>) from water and sediments of Meiliang Bay and from the center of Lake Taihu were tested from January to June, 2017. Indoor <i>Microcystis aeruginosa</i> was used as the control group. The kit for total RNA extraction in <i>Microcystis</i> was optimized. Results showed that the optimized kit extracted high-concentrations and high-quality total RNA from <i>Microcystis</i>. The extraction purity and concentration were significantly higher than those extracted by the original kit. The transcription level of <i>gvpC</i> increased gradually until a2019-01-01T00:00:00Z20192026-04-29T03:22:58.606Z2019-07-25T07:00:25ZS-EPMC65994503128970110.7717/peerj.7188