{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang RH"],"funding":["the China 13th Five-Year National Science and Technology Major Project and the key R & D Program of Hunan Province","China Mega-Projects for Infectious Disease"],"pagination":["86"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6604330"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["BACKGROUND:Human adenoviruses are a common group of viruses that cause acute infectious diseases. Human adenovirus (HAdV) 3 and HAdV 7 cause major outbreaks of severe pneumonia. A reliable and practical method for HAdV typing in clinical laboratories is lacking. A simple, rapid and accurate molecular typing method for HAdV may facilitate clinical diagnosis and epidemiological control. METHODS:We developed and evaluated duplex real-time recombinase-aided amplification (RAA) assays incorporating competitive internal controls for detection of HAdV 3 and HAdV 7, respectively. The assays were performed in a one-step in a single tube reaction at 39° for 20 min. RESULTS:The analytical sensitivities of the duplex RAA assays for HAdV 3 and HAdV 7 were 5.0 and 14.8 copies per reaction, respectively "],"journal":["Virology journal"],"pubmed_title":["Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7."],"pmcid":["PMC6604330"],"funding_grant_id":["2018ZX10713002 and 2017SK2101","2018ZX10711001, 2017ZX10104001 and 2018ZX10713-002"],"pubmed_authors":["Xiang XY","Wang RH","Fan GH","Zhan ZF","Chen ZW","Zhang Y","Li XN","Zhang H","Ma XJ","Shen XX","Qi JJ"],"additional_accession":[]},"is_claimable":false,"name":"Development and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7.","description":"BACKGROUND:Human adenoviruses are a common group of viruses that cause acute infectious diseases. Human adenovirus (HAdV) 3 and HAdV 7 cause major outbreaks of severe pneumonia. A reliable and practical method for HAdV typing in clinical laboratories is lacking. A simple, rapid and accurate molecular typing method for HAdV may facilitate clinical diagnosis and epidemiological control. METHODS:We developed and evaluated duplex real-time recombinase-aided amplification (RAA) assays incorporating competitive internal controls for detection of HAdV 3 and HAdV 7, respectively. The assays were performed in a one-step in a single tube reaction at 39° for 20 min. RESULTS:The analytical sensitivities of the duplex RAA assays for HAdV 3 and HAdV 7 were 5.0 and 14.8 copies per reaction, respectively ","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jul","modification":"2025-05-29T21:46:01.204Z","creation":"2019-07-25T07:02:19Z"},"accession":"S-EPMC6604330","cross_references":{"pubmed":["31262315"],"doi":["10.1186/s12985-019-1178-9"]}}