biostudies-literatureWang RHthe China 13th Five-Year National Science and Technology Major Project and the key R & D Program of Hunan ProvinceChina Mega-Projects for Infectious Disease86https://www.ebi.ac.uk/biostudies/studies/S-EPMC6604330biostudies-literatureUnknown16(1)BACKGROUND:Human adenoviruses are a common group of viruses that cause acute infectious diseases. Human adenovirus (HAdV) 3 and HAdV 7 cause major outbreaks of severe pneumonia. A reliable and practical method for HAdV typing in clinical laboratories is lacking. A simple, rapid and accurate molecular typing method for HAdV may facilitate clinical diagnosis and epidemiological control. METHODS:We developed and evaluated duplex real-time recombinase-aided amplification (RAA) assays incorporating competitive internal controls for detection of HAdV 3 and HAdV 7, respectively. The assays were performed in a one-step in a single tube reaction at 39° for 20 min. RESULTS:The analytical sensitivities of the duplex RAA assays for HAdV 3 and HAdV 7 were 5.0 and 14.8 copies per reaction, respectively Virology journalDevelopment and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7.PMC66043302018ZX10713002 and 2017SK21012018ZX10711001, 2017ZX10104001 and 2018ZX10713-002Xiang XYWang RHFan GHZhan ZFChen ZWZhang YLi XNZhang HMa XJShen XXQi JJfalseDevelopment and evaluation of recombinase-aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7.BACKGROUND:Human adenoviruses are a common group of viruses that cause acute infectious diseases. Human adenovirus (HAdV) 3 and HAdV 7 cause major outbreaks of severe pneumonia. A reliable and practical method for HAdV typing in clinical laboratories is lacking. A simple, rapid and accurate molecular typing method for HAdV may facilitate clinical diagnosis and epidemiological control. METHODS:We developed and evaluated duplex real-time recombinase-aided amplification (RAA) assays incorporating competitive internal controls for detection of HAdV 3 and HAdV 7, respectively. The assays were performed in a one-step in a single tube reaction at 39° for 20 min. RESULTS:The analytical sensitivities of the duplex RAA assays for HAdV 3 and HAdV 7 were 5.0 and 14.8 copies per reaction, respectively 2019-01-01T00:00:00Z2019 Jul2025-05-29T21:46:01.204Z2019-07-25T07:02:19ZS-EPMC66043303126231510.1186/s12985-019-1178-9